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EMAGE:4240

Gad1 glutamic acid decarboxylase 1 ( MGI:95632)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4240
Fig1B. Copyright: This image is from Maddox DM, BMC Dev Biol 2001;1(1):1, an open-access article, licensee BioMed Central Ltd.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4240Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4240_wholemount_strong_3D_1.wlz
4240_wholemount_moderate_3D_1.wlz
4240_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4240_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail
detected detected
regionalExpression is localized towards the tip of the tail.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3653517
Entity Detected:Gad1, glutamic acid decarboxylase 1 ( MGI:95632)
Sequence:sense strand is shown

>MGI:3653517
CCAAGACCACCGAGCTGATGGCATCTTCCACTCCTTCGCCTGCAACCTCCTCGAACGCGGGAGCGGATCC
TAATACTACCAACCTGCGCCCTACAACGTATGATACTTGGTGTGGCGTAGCCCATGGATGCACCAGAAAA
CTGGGCCTGAAGATCTGTGGCTTCTTACAAAGGACCAATAGCCTGGAAGAGAAGAGTCGTCTTGTGAGCG
CCTTCAGGGAGAGGCAGTCCTCCAAGAACCTGCTTTCCTGTGAAAACAGTGACCAGGGTGCCCGCTTCCG
GCGCACAGAGACCGACTTCTCCAACCTGTTTGCTCAAGATCTGCTTCCAGCTAAGAACGGGGAGGAGCAA
ACTGCGCAGTTCTTGCTGGAAGTGGTAGACATACTCCTCAACTATGTCCGCAAGACATTTGATCACTCCA
CCAAGGTTCTGGATTTCCACCACCCACACCAGTTGCTGGAAGGCATGGAAGGCTTTAATTTGGAGCTGTC
TGACCACCCCGAGTCTCTGGAGCAGATCCTGGTTGACTGTAGAGACACCCTGAAGTACGGGGTTCGCACA
GGTCACCCTCGATTTTTCAACCAGCTCTCTACTGGTTTGGATATCATTGGTTTAGCTGGTGAATGGCTGA
CATCGACTGCCAATACCAATATGTTCACATATGAAATTGCACCCGTGTTTGTTCTCATGGAACAGATTAC
TCTTAAGAAGATGAGAGAGATCGTTGGATGGTCAAATAAAGATGGTGATGGGATATTTTCTCCTGGGGGA
GCCATATCCAATATGTACACGATCATGGCTGCTCGTTACAAGTACTTCCCAGAAGTGAAGACAAAAGGCA
TGGCGGCTGTGCCCAAACTGGTCCTCTTCACCTCAGAACACAGTCACTATTCCATAAAGAAAGCCGGGGC
TGCGCTTGGCTTTGGAACGGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAGATAATTCCGGCT
GATTTAGAGGCAAAAATTCTTGATGCCAAACAAAAGGGCTATGTTCCCCTTTATGTCAATGCAACCGCAG
GCACGACTGTTTACGGAGCATTCGATCCAATCCAGGAAATTGCGAGCATATGTGAGAAATACAACCTTTG
GCTGCATGTGGATGCTGCCTGGGGTGGTGGACTGCTCATGTCCCGGAAGCACCGCCACAAACTCAGCGGC
ATAGAAAGGGCCAATTCAGTCACCTGGAACCCTCACAAGATGATGGGCGTGCTGCTCCAGTGCTCTGCCA
TTCTGGTCAAGGAAAAGGGTATACTCCAAGGATGCAACCAGATGTGTGCAGGCTACCTCTTCCAGCCAGA
CAAGCAGTATGACGTCTCCTATGACACCGGGGACAAGGCGATTCAGTGTGGCCGCCATGTGGACATCAAC
AAGTTCTGGCTGATGTGGAAAGCAAAGGGCACCGTGGGATTTGAAAACCAGATCAACAAATGCCTGGAGC
TGGCTGATTACCTCTACGCCAAGATTAAAAACAGAGAAGAGTTTGAGATGGTTTTCGATGGTGAGCCTGA
GCACACAAATGTCTGTTTCTGGTACATTCCACAAAGCCTTCGAGGGGTTCCAGATAGCCCTGAGCGACGA
GAAAAGCTACACAGGGTGGCTCCCAAGATCAAAGCTCTGATGATGGAGTCAGGAACAACCATGGTGGCAT
ACCAGCCTCAAGGGGACAAGGCCAACTTCTTCCGGATGGTCATCTCTAACCCAGCCGCCTCCCAGTCTGA
CATCGATTTCCTCACCGAGGAGATAGAGAGGTTGGGTCAGGATCTGTAATCCCTCTTTACAGAACCAGAA
TCATCGGCCATGCTTGTGCCCCTCTGGTACCCTAGAGCACAGCTCTGTCAGTAGCTGACACATCTAGGCC
ATCTCACTGAAGGAAATTACAATCTCTTAAAGAATATTTGTCACATTT
nt 104 - nt 2041 of Z49976.1
Notes:The Gad1 probe used in this study by Maddox & Condie, 2001 [PMID:11178105] is described as follows: "The Gad1 probe was derived from an EST clone (accession W59173). Its 5' end corresponds to nucleotide 142 in exon 1 (Szabo et al., 1996 [PMID:8985122] ) and the 3' end is at nucleotide 2041 in the cDNA sequence (Katarova et al., 1990" [PMID:12106047] , Z49976.1). Editors note: Pair-wise BLAST of W59173 against Z49976.1 places the 5' end of the template fragment at position 104 in Z49976.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Maddox DM; Condie BG, 2001 [PMID:11178105] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1089/dna.1996.15.1081] [ PMID:8985122] Szabo G, Katarova Z, Kortvely E, Greenspan RJ, Urban Z 1996 Structure and the promoter region of the mouse gene encoding the 67-kD form of glutamic acid decarboxylase. DNA Cell Biol (15):1081-91
 [ doi:10.1111/j.1460-9568.1990.tb00412.x] [ PMID:12106047] Katarova Z, Szabo G, Mugnaini E, Greenspan RJ 1990 Molecular Identification of the 62 kd Form of Glutamic Acid Decarboxylase from the Mouse. Eur J Neurosci (2):190-202
 [ doi:10.1186/1471-213X-1-1] [ PMID:11178105] Maddox DM, Condie BG. 2001 Dynamic expression of a glutamate decarboxylase gene in multiple non-neural tissues during mouse development. BMC developmental biology (1)
Links:MGI:3653586 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI