EMAGE mouse gene expression spatial database

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EMAGE:4375

Map3k5 mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
TS16 (10.5 dpc)
in situ hybridisation

Data Images


Fig4C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.05.001] Gene Expr Patterns 7: 808-16, Ferrer-Vaquer A; Maurey P; Firnberg N; Leibbrandt A; Neubuser A, Expression of ASK1 during chick and early mouse development. Copyright 2007	Fig4C'''. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.05.001] Gene Expr Patterns 7: 808-16, Ferrer-Vaquer A; Maurey P; Firnberg N; Leibbrandt A; Neubuser A, Expression of ASK1 during chick and early mouse development. Copyright 2007	Fig4C''. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.05.001] Gene Expr Patterns 7: 808-16, Ferrer-Vaquer A; Maurey P; Firnberg N; Leibbrandt A; Neubuser A, Expression of ASK1 during chick and early mouse development. Copyright 2007	Fig4C'. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.05.001] Gene Expr Patterns 7: 808-16, Ferrer-Vaquer A; Maurey P; Firnberg N; Leibbrandt A; Neubuser A, Expression of ASK1 during chick and early mouse development. Copyright 2007

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Figs4C', 4C'' and 4C''' are sections from teh approximate regions indicated in Fig4C. Image annotations: as - aortic sac; bc - bulbus cordis; lp - lens placode; ma - mandibular arch; ot - otocyst; ph - pharynx; v - ventricle.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4375_wholemount_strong_3D_1.wlz

4375_wholemount_moderate_3D_1.wlz

4375_wholemount_weak_3D_1.wlz

4375_wholemount_possible_3D_1.wlz

4375_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4375_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

strong

regional

Expression is in the body wall enclosing the heart and nearby organs.

1st branchial arch mandibular component ectoderm

detected

1st branchial arch mandibular component mesenchyme

detected

1st branchial arch maxillary component ectoderm

detected

2nd branchial arch mesenchyme

weak

3rd branchial arch ectoderm

weak

3rd branchial arch mesenchyme

weak

4th branchial arch ectoderm

weak

4th branchial arch mesenchyme

weak

2nd branchial arch ectoderm

weak

lens pit

detected

otocyst

detected

dorsal mesocardium

detected

primitive ventricle

detected

primitive ventricle cardiac muscle

detected

outflow tract

detected

regional

Expression is in the the aortic sac and the truncus arteriosus.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3720337
Entity Detected:	Map3k5, mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
Sequence:	sense strand is shown >MGI:3720337 GAAATCCCAGAGAGAGACAGCAGATACTCTCAGCCCCTGCATGAAGAAATCGCCCTGCACAAGCATCTGA AACACAAAAACATTGTCCAGTATCTGGGCTCCTTCAGTGAGAACGGCTTCATCAAAATCTTCATGGAGCA AGTCCCAGGAGGAAGCCTCTCTGCTCTCCTTCGTTCAAAATGGGGCCCATTAAAGGACAACGAACAGACG ATTGGCTTCTATACGAAGCAGATACTGGAAGGATTAAAATACCTTCACGACAATCAGATAGTCCACCGGG ATATAAAGGGTGACAATGTATTGATTAATACCTACAGTGGTGTGCTCAAGATCTCTGACTTCGGGACATC CAAGAGGCTTGCTGGCATAAACCCATGTACCGAAACCTTTACAGGTACCCTTCAGTATATGGCGCCAGAA ATAATCGATAAAGGACCACGAGGCTATGGAAAGGCCGCCGACATTTGGTCACTGGGCTGCACAATCATCG AAATGGCTACTGGAAAACCACCATTTTATGAACTAGGAGAGCCACAGGCAGCCATGTTCAAGGTGGGGAT GTTTAAGGTCCACCCGGAGATCCCAGAGTCCATGTCGGCGGAGGCCAAGGCGTTCATACTGAAGTGTTTT GAACCAGACCCTGACAAGAGAGCCTGTGCTAATGACTTGCTTATTGATGAGTTCTTAAAAGTGTCAAGCA AAAAGAAAAAGACACAACCCAAACTTTCAGCTCTCTCGACTGGATCGAATGAGTATCTTAGAAGCATCTC CCTGCCGGTCCCTGTCCTGGTAGAAGACACCAGCAGCAGTAGCGAGTATGGCTCTGTTTCTCCTGATACA GAGCTGAAGGCGGACCCCTTCTCCTTCAAAGCCAGAGCTAAGTCCTGTGGAGAAAAGGACGGGAAAGGGA TACGGACATTGTTCCTGGGCATTCCAGATGAAAATTTTGAAGATCACAGTGCGCCCCCATCTCCTGAAGA GAAAGACTCGGGCTTTTTTATGCTGAGAAAGGATAGTGAGAGGCGAGCTACCCTTCACAGAATCCTGACC GAAGACCAGGACAAGGTTGTAAGGAACCTAATGGAATCTCTGGCCCAGGGTGCTGAAGAGCCTAAACTAA ACTGGGAACACATCACAACCCTCATCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGAAAAATCAT AGCCAC
	nt 2281 - nt 3476 of AB006787.3
Notes:	The Map3k5 (Ask1) probe template used in this study by Ferrer-Vaquer et al., 2007 [PMID:17602894] is described as follows: Mouse Ask1 was cloned from adult kidney cDNA by PCR amplification using the following primers: 5'-GAAATCCCAGAGAGAGACGCA-3' and 5'-GTGGCTATGATTTTTCGGTCAGT-3'. The resulting 1195 bp long PCR product was cloned using the TopoTA cloning system (Invitrogen).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	10.5 dpc
Theiler Stage:	TS16
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Ferrer-Vaquer A; Maurey P; Firnberg N; Leibbrandt A; Neubuser A, 2007 [PMID:17602894] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2007.05.001] [ PMID:17602894] Ferrer-Vaquer A, Maurey P, Firnberg N, Leibbrandt A, Neubuser A 2007 Expression of ASK1 during chick and early mouse development. Gene Expr Patterns (7):808-16
Links:	MGI:3720343 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source