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EMAGE:4390

Ano1 anoctamin 1, calcium activated chloride channel ( MGI:2142149)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4390 EMAGE:4390
Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.08.004] Gene Expr Patterns 8: 19-26, Rock JR; Cecilia Lopez M; Baker HV; Harfe BD, Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Copyright 2007 Fig4C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.08.004] Gene Expr Patterns 8: 19-26, Rock JR; Cecilia Lopez M; Baker HV; Harfe BD, Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Copyright 2007

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig4A - forelimb. Fig4C - hindlimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:4390Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4390_wholemount_strong_3D_1.wlz
4390_wholemount_moderate_3D_1.wlz
4390_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4390_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regionalExpression is in the posterior fore- and hindlimb mesenchyme.
forelimb bud mesenchyme
detected detected
regionalExpression is in the posterior fore- and hindlimb mesenchyme
hindlimb bud mesenchyme
detected detected
regionalExpression is in the posterior fore- and hindlimb mesenchyme
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1406293
Entity Detected:Ano1, anoctamin 1, calcium activated chloride channel ( MGI:2142149)
Sequence:sense strand is shown

>MGI:1406293
CCCACGCGTCCGGTCGCCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCC
GCTCTTTCCGGATGGAGGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCAT
TATGCTGGGCAAGCAGCTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATC
CGCTACCTGAAGCTGCGCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATG
AGGTGGACTTCAACCTCGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGG
CTTTGTCACCCTGTTTGTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAG
ATCCGCCTGGATGCCAAAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCG
GCATCTGGTATAACATCCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTT
CACGTCTGACTTCATCCCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTC
GTCAATCACACGCTCTCTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGG
ACCTGGGCTATGAGGTTCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTA
TGACATCTCCAAAGACTTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTG
GTGATGTTCATGAGTGACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCC
ACAAAGAGAAGGTTCTCATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACAC
ATGGATGGAGAAGGAGAAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCA
GGCGACGGCAGCCCAGTCCCCAGCTACGAGTACCATGGGGACGCACTGTAGCCATGATGACAGGCCAGCA
GGCCAGCAGGCTTCTGACCATCACCAAGTGACCACTCGGCTTCCTCCAAAGCCAGCACTTCTAGCCCCAC
CAGGGCCTATGGCTCTTGTGTCGTCTGGACGTGTGGAGGAGCACTCTGATGCGACAACGGTCTTCTTTCT
TCTTTCTGTTTCTTCTCATTTTTGCACAAGGCCATTATGCAGGGAAGTTTTTATCTTTAGTATTCAATGC
CAAGGATGGCTGGTGATGGGGATGAACAGGGTGAGGCATGTTCCACAGACATATGGAGGAATATAGAGCC
CCCCTGTGGTGGTAGGGAGCCAAGTCAGCATCCTTAGTCTGAAGGATGCCCTGGGATAGATGCAGAATGG
TACCTAGGAACCTGCTGACATTTTGCAGAAGGGTGAACAACTCAGACTCAGATTCCCAAAGATGTCACCT
GCTCTAGCTGGACAAGCTGGAAGACTCCACTGTCTTTCCTTCTGGGAGCTCAGAAGTTCAGACCTGGGGT
CAGAAACAGAGGTGCCTGACCACCCACACAGCACACACACATGGTGGCTTCACATTGTACTCCAGTAACG
GAGGAGTACAGGGGCTCCCAGTTGTCCAGTACCCTTGGGGTCCCTGGCAGGCTGCACACAAGCCACTCAA
GGGCCCCTCACATGAAGCTGATGTCCTCCTTGTGAGACAAGCTGTCGTGGCAATCTCTAAACAGGCTGGA
GGGCTTCACCAAGCCCTGGGGCCTCTGTGGGTTCATTTTTTTTTTTTTTTGCTTTTTAAACCATCTATTG
GTTTTTGTCTAAAAATCAATAATGTGATGGATTTGAGGGATTTTCTTTCTGTAGCTGAAGGTGGAAGGAG
TTTATTTCATTAGTTAACCAAATACTGAGAGGAAATTAAAATATCATTACTACCAAAGATTTTTCTCAAT
AAAAGCTTCTATTTTGGTAACACACCTATATTTTCACTCGTGGGGATGAGACCCTCAGCACTTACCATTG
TAATAGCTTCTAAAACAAGCCTCATGGGCCAGCTTCATTTGATGTGCTAGCCAGCTGTGCAAGGCCTCTC
GGCACCATTGCCAGAAGTGTCTCTCTCGCCCAAGCCGAAGAAGGCAATTCCCTGCACTCGTCTTAGGGCA
AGGCAGACAGTGCTGTAGTTCGTGAGCTGGGGTCCGAATCTCAGGCTTCCAAGGTTTGCACTGTTTGCTA
AAATCATGAGACCTGATGCTTCTATTTTCTCTTGTGCAGTGATGTGGTTTCCAATGATGTTTTGTATATA
GCTACTGTATCCAAAATTAGATAATTACCAAAAATTCTTTTACAGAAACCATTTTTAAAAAACAACAAAA
AAAACTAAAAAAAAAAAAAAAAA
nt 1 - nt 2473 of BC006062.1
Notes:The Tmem16a probe used in this study by Rock et al., 2007 [PMID:17911046] was generated from BC006062(.1) - see Table 1 therein.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Rock JR; Cecilia Lopez M; Baker HV; Harfe BD, 2007 [PMID:17911046] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2007.08.004] [ PMID:17911046] Rock JR, Lopez MC, Baker HV, Harfe BD 2007 Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Gene Expr Patterns (8):19-26
Links:MGI:3764534 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI