EMAGE mouse gene expression spatial database

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EMAGE:4395

Lrrtm3 leucine rich repeat transmembrane neuronal 3 ( MGI:2389177)
TS15 (10.0 dpc)
in situ hybridisation

Data Images


Fig3I. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2006.05.004] Gene Expr Patterns 7: 23-9, Haines BP; Rigby PW, Developmentally regulated expression of the LRRTM gene family during mid-gestation mouse embryogenesis. Copyright 2007

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: nt - neural tube; arrowhead - presomitic mesoderm/somite boundary.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4395_wholemount_strong_3D_1.wlz

4395_wholemount_moderate_3D_1.wlz

4395_wholemount_possible_3D_1.wlz

4395_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4395_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural tube

detected

regional

Expression is in the rostral neural tube.

future hindbrain

weak

future forebrain

detected

eye

detected

tail paraxial mesenchyme

detected

regional

Expression is at the presomitic mesoderm/somite boundary.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3694248
Entity Detected:	Lrrtm3, leucine rich repeat transmembrane neuronal 3 ( MGI:2389177)
Sequence:	sense strand is shown >MGI:3694248 GATCCGAAGTTTAGCCAGGAATGTCTTTGCTGGCATGATCAGACTCAAAGAGCTTCACCTGGAGCACAAT CAATTTTCCAAGCTCAACCTGGCCCTGTTCCCAAGGCTGGTGAGCCTTCAGAACTTGTACATGCAGTGGA ATAAAATCAGTGTCATAGGGCAAACCATGTCCTGGACCTGGAGTTCCTTACAGAGGCTCGACCTGTCTGG TAATGAGATCGAAGCCTTCAGCGGACCCAGTGTCTTCCAGTGTGTTCCCAATCTGCAACGCCTCAACCTG GATTCCAACAAGCTCACATTTATTGGACAAGAGATTCTGGATTCTTGGATCTCTCTCAATGACATCAGTC TGGCTGGGAATATATGGGAATGCAGCAGGAATATCTGTTCCCTGGTAAACTGGCTGAGAAGTTTTAAAGG TCTGAGAGAGAATACAATCATCTGCGCCAGTCCCAAAGAGCTGCAGGGGGTTAACGTGATCGATGCGGTG AAGAACTACAGCATCTGTGGGAAGAGCACTACCACAGAGAGGTTTGACCTGGCCAGGGCCCTCCCCAAGC CCACATTTAAGCCCAAGCTCCCCAGGCCGAAGCACGAGAGCAAGCCTCCTCTGCCCCCCACGGTGGGAGC TACCGAGCCCAGCCCAGAGACAGATGTGGACACGGAGCACATCTCCTTCCATAAGATCATCGCGGGCAGC GTGGCCCTTTTCCTGTCGGTGCTGGTCATCCTCCTGGTGATGTACGTGTCCTGGAAGCGGTACCC
	nt 1118 - nt 1882 of NM_178678.4
Notes:	The Lrrtm3 probe used in this study by Haines & Rigby, 2007 [PMID:16860615] is described as follows: "A LRRTM3 cDNA was obtained from IMAGE clone 5359182 digested with KpnI and BamHI. The fragment equivalent to bases 1118 to 1882 of LRRTM3 sequence NM_178678 was cloned into KpnI and BamHI digested pBluescript II KS. An antisense probe was generated by digesting with BamHI and transcribing with T3 polymerase. "
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.0 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + NBT/Red Phos

General Information

Authors:	Haines BP 7 Rigby PW, 2007 [PMID:16860615] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2006.05.004] [ PMID:16860615] Haines BP, Rigby PW 2007 Developmentally regulated expression of the LRRTM gene family during mid-gestation mouse embryogenesis. Gene Expr Patterns (7):23-9
Links:	MGI:3694268 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source