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EMAGE:4581

Fgf4 fibroblast growth factor 4 ( MGI:95518)
TS17 (10.75 dpc)
in situ hybridisation

Data Images
EMAGE:4581
Fig5A. Copyright: This image is from Haramis AG, Development 1995 Dec;121(12):4237-45, [PMID:8575323] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4581Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4581_wholemount_notDetected.wlz
4581_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4581_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud apical ectodermal ridge
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17265
Entity Detected:Fgf4, fibroblast growth factor 4 ( MGI:95518)
Notes:The Fgf4 probe used in this study by Haramis et al., 1995 [PMID:8575323] is indicated as that originally described by Niswander & Martin, 1992 [PMID:1618140] . However, two Fgf4 probes were used by Niswander & Martin, each giving "identical results": the "full length coding sequence (see Hebert et al, 1990 [PMID:2318343] " and "the 3' untranslated region from nt 3630-3819 (see Brookes et al, 1989 [PMID:2740210] ". Unable to ascertain which was used here.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 101/Rl * C3H/Rl
Age:10.75 dpc
Theiler Stage:TS17
Mutations:Fmn1ld-Is(17;In2)1Gso/Fmn1+
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Haramis AG; Brown JM; Zeller R, 1995 [PMID:8575323] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8575323] Haramis AG, Brown JM, Zeller R 1995 The limb deformity mutation disrupts the SHH/FGF-4 feedback loop and regulation of 5' HoxD genes during limb pattern formation. Development (121):4237-45
 [ PMID:1618140] Niswander L, Martin GR 1992 Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development (114):755-68
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
 [ doi:10.1093/nar/17.11.4037] [ PMID:2740210] Brookes S, Smith R, Thurlow J, Dickson C, Peters G 1989 The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2. Nucleic Acids Res (17):4037-45
Links:MGI:1336034 same experiment
 MGI:1856338 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI