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EMAGE:4642

Adm adrenomedullin ( MGI:108058)
TS15 (9.5 dpc)
in situ reporter

Data Images
EMAGE:4642
Fig 2B. Copyright: This image is from Caron & Smithies, Proc Natl Acad Sci U S A 2001 Jan 16;98(2):615-619. [PMID:11149956] Copyright 2001 National Academy of Sciences, U.S.A.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: heart (arrowhead h), developing vasculature (arrowhead v), forebrain (arrowhead b); a speck of nonspecific fluorescence has been covered with a black box.
Expression Pattern Description
Spatial Annotation:
EMAGE:4642Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4642_wholemount_strong.wlz
4642_wholemount_moderate.wlz
4642_wholemount_possible.wlz
4642_wholemount_notDetected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4642_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
moderate moderate
cardiovascular system
strong strong
There is strong expression in the developing vasculature.
future forebrain
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Adm, adrenomedullin ( MGI:108058)
Notes:Adm expression was detected in this study by Caron & Smithies, 2001 [PMID:11149956] thus: An in-frame EGFP gene and a neomycin resistance cassette were inserted into exon 2, and a genomic fragment corresponding to exons 3 and 4 was deleted. RT-PCR analysis on embryonic RNA of homozygotes demonstrated that no detectable Adm transcript was expressed from this allele. Full information on the production of the line is given in Figure 1 of the reference.
Labelled with:GFP
Specimen
Organism:mouse
Strain:involves: 129S6/SvEvTac
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Admtm1Unc/Adm+
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Caron KM; Smithies O, 2001 [PMID:11149956] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.021548898] [ PMID:11149956] Caron KM, Smithies O 2001 Extreme hydrops fetalis and cardiovascular abnormalities in mice lacking a functional Adrenomedullin gene. Proc Natl Acad Sci U S A (98):615-9
Links:MGI:3052467 same experiment
 MGI:2176598 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI