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EMAGE:478

T brachyury ( MGI:98472)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:478
Figure 7 of Wilson et al., 1993 [PMID:8404534] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This image shows 9 wholemount stained tails.
Expression Pattern Description
Spatial Annotation:
EMAGE:478Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
478_wholemount_strong_3D_1.wlz
478_wholemount_moderate_3D_1.wlz
478_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:478_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail
strong strong
notochord
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334985
Entity Detected:T, brachyury ( MGI:98472)
Sequence:sense strand is shown

>MGI:1334985
GGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGG
AGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAA
GAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAG
GGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGC
TAACTAACGAGATGATTGTGACCAAGAACGGCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGG
CCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATAT
GTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAG
ACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAA
GCTCAATGGAGGGGGACAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGA
GTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCT
ACCAGAATGAGGAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAA
AGAAAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGG
GGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGC
TCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCC
CAGCCCCTATGCTCATCGGAACAGCTCTCCAACCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTG
CAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACG
CCAGCCCACCTACTGGCTCTAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGG
CTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACA
CCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTA
CAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGT
GTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGA
TCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACA
ACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCT
ACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGA
CTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGA
CTTTGAAATCCTGGAATTCGTCCACCCCCTGTCCTACTTTAGTGAGACACAAGGTACACCTCTAATGTCC
TCCCTTGTTGCCTTAGAGTAGTTAACTTTGAGGACAGAAAAAAGCATAGCCAGAAGATTGTAACTGAACC
GTCAACTGTTCTGCCCTTGGAACATGCCTACTTTAAGCACACGTAGCTTTTTGTGTTGGGAAGTCAACTG
TATGGATACTTTTCTGTTGACAAAGTAGCCAAAGACAATCTGCAGAAAGTGTTTTCTGCATAATAAAGGC
AATATATAGCACCTGG
nt 1 - nt 2046 of X51683.1
Notes:The T probe used in this study by Wilson et al., 1993 [PMID:8404534] is described as "an antisense digoxigenin-labelled riboprobe transcribed from the full-length T cDNA (gift of Dr B. Herrmann)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129/Sv
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Wilson et al., 1993 [PMID:8404534] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8404534] Wilson V, Rashbass P, Beddington RS 1993 Chimeric analysis of T (Brachyury) gene function. Development (117):1321-31
 [ doi:10.1038/343617a0] [ PMID:2154694] Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H 1990 Cloning of the T gene required in mesoderm formation in the mouse. Nature (343):617-22
Links:MGI:1343043 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI