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EMAGE:479

Scx scleraxis ( MGI:102934)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:479
Fig 8a, Leitges et al, 2000 [PMID:10804169] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrows point to mesenchymal anlagen of pedicles.
Expression Pattern Description
Spatial Annotation:
EMAGE:479Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
479_wholemount_strong_3D_1.wlz
479_wholemount_moderate_3D_1.wlz
479_wholemount_possible_3D_1.wlz
479_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:479_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial skeleton
detected detected
regionalExpression is detected in the mesenchymal anlagen of the pedicles and ribs
rib pre-cartilage condensation
detected detected
regionalExpression is detected in the mesenchymal anlagen of the pedicles and ribs
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:24254
Entity Detected:Scx, scleraxis ( MGI:102934)
Sequence:sense strand is shown

>MGI:24254
CTCGGCTGGCGGCCCCACTCCAGTCCGAACACATGTGCCCGCCTCACCCGGCAGCCGCCACCGCGGGGCG
CAGCGGAGACCCCTGGCCCGGCGGCCTGTGGGGACCTAAAGAGGCGGCATGAGCAGCGCACGGTGGAGCT
GACGCCGCGCCCCCTGCCCGGCCCATGTCCTTCGCCATGCTGCGTTCAGCGCCGCCGCCGGGTCGCTACC
TGTACCCTGAGGTGAGCCCGCTGTCGGAGGATGAGGACCGCGGAAGCGAGAGCTCGGGCTCCGACGAGAA
ACCCTGCCGTGTGCATGCTGCGCGCTGTGGCCTCCAGGGCGCCCGGCGGCGGGCAGGAGGACGGAGGGCC
GCGGGTAGCGGGCCAGGACCCGGGGGGCGGCCAGGCCGCGAGCCCCGGCAGCGGCACACAGCGAATGCGC
GCGAGCGGGACCGCACCAACAGCGTGAACACGGCCTTCACTGCGCTGCGCACACTCATCCCCACCGAGCC
AGCGGACCGCAAGCTCTCCAAGATTGAGACGCTGCGCCTGGCCTCCAGCTACATTTCTCACCTGGGCAAT
GTGCTGCTGGTGGGTGAGGCCTGTGGCGACGGGCAACCATGCCACTCGGGACCCGCTTTCTTCCACAGCG
GTCGTGCGGGCAGCCCACTGCCACCGCCGCCGCCGCCACCACCACTGGCCAGAGACGGCGGCGAGAACAC
CCAGCCCAAACAGATCTGCACCTTCTGCCTCAGCAACCAGAGAAAGTTGAGCAAAGACCGTGACAGAAAG
ACGGCGATTCGAAGTTAGAAGGAGGAGGGTCCACAGCAGCCAAGAGGTGATGCCACTAGTGAAGGTGGAT
GCCACTGAAGAGTCACGGAGAGCCCCACTTTGGACCTGATCTGAGCGCAGGTCCCCAAGAGCACGCTCAC
CAGGCCAGACCTGCCCCAGCTATGAGCAGGGCCTATGAACAGAGAGATGGACAGGCGGGTGATGGCTTGA
CTCTAAGCAGCCGAACACTGACCCAGGCCCACTGGGACTTTCCATGCTGGCTCTCTACCTGGGCCAAGTG
TTGGCATCTTGTGTCTTTAATATGATAATATAAAGTCTGGAAATTTTGTATAATTAAAAATGAAACAGTA
TCTTCCAAAAAAAAAAAAAA
nt 1 - nt 1140 of S78079.1
Notes:The Scx probe used in this study by Leitges et al, 2000 [PMID:10804169] is indicated as that described by Cserjesi et al, 1995 [PMID:7743923] . ie "a 1.2-kb scleraxis cDNA insert cloned into pBSKII+". Sequence S78079 above was submitted by Cserjesi in conjunction iwth the above publication.
Chemistry:RNA
Strand:antisense
Label:undefined
Specimen
Organism:mouse
Strain:NMRI
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:undefined
General Information
Authors:Leitges et al, 2000 [PMID:10804169] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10804169] Leitges M, Neidhardt L, Haenig B, Herrmann BG, Kispert A 2000 The paired homeobox gene Uncx4.1 specifies pedicles, transverse processes and proximal ribs of the vertebral column. Development (127):2259-67
 [ PMID:7743923] Cserjesi P, Brown D, Ligon KL, Lyons GE, Copeland NG, Gilbert DJ, Jenkins NA, Olson EN 1995 Scleraxis: a basic helix-loop-helix protein that prefigures skeletal formation during mouse embryogenesis. Development (121):1099-110
Links:MGI:1859385 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI