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EMAGE:492

Wnt1 wingless-related MMTV integration site 1 ( MGI:98953)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:492
Figure 5.K, Suda et al., 1999 [PMID:9895322] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:492Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
492_wholemount_strong_3D_1.wlz
492_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:492_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalExpression extends from the isthmus to the caudal diencephalon
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328384
Entity Detected:Wnt1, wingless-related MMTV integration site 1 ( MGI:98953)
Sequence:sense strand is shown

>MGI:1328384
ATCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTCCGGGGAGAAGGGGCGGGACCTACGCTTCC
TCATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTGTTCTCTGAGATGCGCCAAGAGTGCAAATG
CCACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGATGCGGCTGCCCACGCTGCGCGCTGTGGGC
GACGTGCTGCGCGACCGCTTCGACGGCGCCTCCCGCGTCCTTTACGGCAACCGAGGCAGCAACCGCGCCT
CGCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCGCACAAGCCTCCCTCCCCTCACGACCTCGT
CTACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCCGCCTGGGCACAGCTGGCACAGCTGGACGA
GCTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCTGCTGTGCTGTGGCCGAGGCCACCGCACGC
GCACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCACTGGTGCTGCCACGTCAGCTGCCGCAACTG
CACGCACACGCGCGTTCTGCACGAGTGTCTATGAGGTGCCGCGCCTCCGGGAACGGGAACGCTCTCTTCC
AGTTCTCAGACACACTCGCTGGTCCTGATGTTTGCCCACCCTACCGCGTCCAGCCACAGTCCCAGGGTTC
ATAGCGATCCATCTCTCCCACCTCCTACCTGGGGACTCCTGAAACCACTTGCCTGAGTCGGCTCGAACCC
TTTTGCCATCCTGAGGGCCCTGACCCAGCCTACCTCCCTCCCTCTTTGAGGGAGACTCCTTTTGCACTGC
CCCCCAATTTGGCCAGAGGGTGAGAGAAAGATTCTTCTTCTGGGGTGGGGGTGGGGAGGTCAACTCTTGA
AGGTGTTGCGGTTCCTGATGTATTTTGCGCTGTGACCTCTTTGGGTATTATCACCTTTCCTTGTCTCTCG
GGTCCCTATAGGTCCCTTGAGTTCTCTAACCAGCACCTCTGGGCTTCAAGGCCTTTCCCCTCCCACCTGT
AGCTGAAGAGTTTCCGAGTTGAAAGGGCACGGAAAGCTAAGTGGGAAAGGAGGTTGCTGGACCCAGCAGC
AAAACCCTACATTCTCCTTGTCTCTGCCTCGGAGCCATTGAACAGCTGTGAACCATGCCTCCCTCAGCCT
CCTCCCACCCCTTCCTGTCCTGCCTCCTCATCACTGTGTAAATAATTTGCACCGAAATGTGGCCGCAGAG
CCA
nt 704 - nt 1966 of M11943.1
Notes:Probe used in this study by Suda et al., 1999 [PMID:9895322] is indicated as being originally described by McMahon & Bradley, 1990 [PMID:2205396] ie. as a "1263bp ClaI-MluI (Figure 2, positions 704-1966 in Fung et al., 1985 [PMID:3018519] ) Wnt-1 cDNA probe".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Suda et al., 1999 [PMID:9895322] , Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9895322] Suda Y, Nakabayashi J, Matsuo I, Aizawa S 1999 Functional equivalency between Otx2 and Otx1 in development of the rostral head. Development (126):743-57
 [ doi:10.1016/0092-8674(90)90385-R] [ PMID:2205396] McMahon AP, Bradley A 1990 The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell (62):1073-85
 [ PMID:3018519] Fung YK, Shackleford GM, Brown AM, Sanders GS, Varmus HE 1985 Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol Cell Biol (5):3337-44
Links:MGI:1328414 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI