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EMAGE:511

T brachyury ( MGI:98472)
TS10 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:511
Fig 8A, Perea-Gomez et al., 1999 [PMID:10498685] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:511Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
511_wholemount_strong_3D_1.wlz
511_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:511_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primitive streak
detected detected
embryo mesoderm
detected detected
Expression is detected in axial mesoderm cells
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2449091
Entity Detected:T, brachyury ( MGI:98472)
Sequence:sense strand is shown

>MGI:2449091
CCTGGAATTCGTCCACCCCCTGTCCTACTTTAGTGAGACACAAGGTACACCTCTAATGTCCTCCCTTGTT
GCCTTAGAGTAGTTAACTTTGAGGACAGAAAAAAGCATAGCCAGAAGATTGTAACTGAACCGTCAACTGT
TCTGCCCTTGGAACATGCCTACTTTAAGCACACGTAGCTTTTTGTGTTGGGAAGTCAACTGTATGGATAC
TTTTCTGTTGACAAAGTAGCCAAAGACAATCTGCAGAAAGTGTTTTCTGCATAATAAAGGCAATATATAG
CACCTGG
nt 1760 - nt 2046 of X51683.1
Notes:Probe used in this study by Perea-Gomez et al., 1999 [PMID:10498685] was originally described by Wilkinson et al., 1990 [PMID:1689462] as corresponding to "residue 1,760 to the end residue in the 3'-untranslated region of T-gene RNA" (see Figure 5 in Herrmann et al., 1990 [PMID:2154694] ). Note the probe sequence also includes a polyA tail which is not included in the sequence link above.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:7.5 dpc
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Perea-Gomez et al., 1999 [PMID:10498685] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10498685] Perea-Gomez A, Shawlot W, Sasaki H, Behringer RR, Ang S 1999 HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo. Development (1999):4499-511
 [ doi:10.1038/343657a0] [ PMID:1689462] Wilkinson DG, Bhatt S, Herrmann BG 1990 Expression pattern of the mouse T gene and its role in mesoderm formation. Nature (343):657-9
 [ doi:10.1038/343617a0] [ PMID:2154694] Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H 1990 Cloning of the T gene required in mesoderm formation in the mouse. Nature (343):617-22
Links:MGI:1346164 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI