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EMAGE:518

Zeb1 zinc finger E-box binding homeobox 1 ( MGI:1344313)
TS20 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:518 EMAGE:518
Figure 2G of Takagi et al., 1998 [PMID:9389660] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 2I of Takagi et al., 1998 [PMID:9389660] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Fig G image annotations: my - migrated myotome; lm - lateral myotome; mm - medial myotome; n - neural tube; asterisks indicate non-specific signals produced by probes trapped in the brain ventricles. Fig I image annotations; A and P indicate the direction of anterior-posterior axis; my - migrated myotome; fl - forelimb bud.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
spinal cord
strong strong
gradedExpression in the neural tube was clearly detected, being particularly prominent in the ventricular zone
tail spinal cord
strong strong
gradedExpression in the neural tube was clearly detected, being particularly prominent in the ventricular zone
tail somite
detected detected
regionalExpression was confined to the myotome and was particularly high in the lateral myotome
forelimb
detected detected
single cellMyotomal deltaEF1 expression was maintained in those cells that migrated in the limb
hindlimb
detected detected
single cellMyotomal deltaEF1 expression was maintained in those cells that migrated in the limb
elbow mesenchyme
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
forearm mesenchyme
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
upper arm mesenchyme
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
knee
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
lower leg mesenchyme
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
upper leg mesenchyme
not detected not detected
deltaEF1 expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1197090
Entity Detected:Zeb1, zinc finger E-box binding homeobox 1 ( MGI:1344313)
Sequence:sense strand is shown

>MGI:1197090
GTCGACAGTCAGTAGCGTTTACCAGAACAGTGTTTATTCTGTCCAGGAAGAACCCTTGAACTTGTCTTGT
GCAAAAAAGGAACCACAAAAGGACAGCTGTGTTACAGACTCAGAACCAGTTGTAAATGTAGTCCCACCAA
GTGCCAACCCCATAAACATTGCTATTCCTACAGTCACTGCCCAGTTACCCACAATCGTGGCCATTGCTGA
CCAGAACAGTGTTCCATGTTTAAGAGCACTGGCCGCCAACAAGCAGACTATTCTGATTCCCCAAGTGGCA
TATACATATTCAGCTACTGTGAGCCCTGCCGTGCAGGAGCCGCCAGTGAAGGTGATCCAGCCAAACGGAA
ACCAGGATGAAAGACAAGACACTAGCTCAGAAGGAGTCTCCACTGTGGAGGACCAGAATGACTCTGACTC
CACGCCACCCAAAAAGAAAACTCGGAAGACAGAGAATGGAATGTATGCATGTGACCTGTGTGACAAGATA
TTTCAGAAGAGCAGCTCACTGTTGAGACACAAATATGAGCACACAGGTAAGAGGCCTCACGAGTGTGGAA
TCTGTAGAAAGGCATTTAAACACAAGCATCATTTGATTGAGCACATGCGGCTGCACTCTGGGGAAAAGCC
CTATCAATGTGACAAGTGTGGCAAGCGCTTCTCACACTCCGGCTCCTACTCTCAACACATGAATCACCGC
TACTCCTACTGCAAGAGAGGAGCTGAAGACAGAGATGCTATGGAGCAGGAAGACGCTGGGCC
nt 2200 - nt 2961 of NM_011546.1
Notes:The Zfhx1a (deltaEF1) probe used in this study by Takagi et al., 1998 [PMID:9389660] is described as a "0.8kb SalI-ApaI cDNA fragment".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Takagi et al., 1998 [PMID:9389660] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9389660] Takagi T, Moribe H, Kondoh H, Higashi Y 1998 DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages. Development (125):21-31
Links:MGI:1306682 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI