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EMAGE:521

Wt1 Wilms tumor 1 homolog ( MGI:98968)
TS24 (16.5 dpc)
in situ hybridisation

Data Images
EMAGE:521
Figure 5I of McCright et al., 2001 [PMID:11171333] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
maturing glomerular tuft
detected detected
regionalExpression in the cup-shaped semicircle of glomerular epithelial cells that are differentiating into podocytes.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2150050
Entity Detected:Wt1, Wilms tumor 1 homolog ( MGI:98968)
Sequence:sense strand is shown

>MGI:2150050
TGTATAGTTAGGACGGAGAGGAGCCTTCCTGCTCAGCTATTCACTCTGAACACTAGCACTGGGCTCTTAA
GAAATGATGTTTTAAGAGCAGAGATCTTTTTTTAATGTCTTTGATTTATTTTTTAGTTGTAATTAGGTAC
ATCCTCAGAGATGTACTTTCCTCCTCTTGTGCAGGATGTGGAGGACTCGTTCCATCATCTGGGGCATCTT
TAGAGTGTATAGACCACACTGGTTATGTGGCTTCAAGTTGTAAAAATTAAAATGACTTTAAAAGAAACTA
GGGGCTGGTCCAGGATCTCACTGGTAAGACTGTTCTTAAGTAACTTAAGTATCTTTGAATCTGCAAGTAT
GTAGGGAAAAAAAAAAAGATATATTATTGTGAGGAAATCCATTGTTTAAAGGTGTGCGTGTGTTGTTGTT
GTTTTTTAAAGGGAGGGAGTTTATTATTTACTGTAGCTTGAAATACTGTGTAAATATATATGTATATATA
TGATGTGCTCTTTGTCAACTAAAATTAGGAGGTGTATGGTATTAGCTGCATCACTGTGTGGATGTCAATC
TTACAGTGTATTGATGATAATACTAAAAATGTAA
nt 2449 - nt 3042 of M55512.1
Notes:The probe used in this study by McCright et al., 2001 [PMID:11171333] is described as "nucleotides 2449-3042 of IMAGE clone M55512".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:16.5 dpc
Theiler Stage:TS24
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:alkaline phosphatase
General Information
Authors:McCright et al., 2001 [PMID:11171333] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171333] McCright B, Gao X, Shen L, Lozier J, Lan Y, Maguire M, Herzlinger D, Weinmaster G, Jiang R, Gridley T 2001 Defects in development of the kidney, heart and eye vasculature in mice homozygous for a hypomorphic Notch2 mutation. Development (128):491-502
Links:MGI:2150128 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI