EMAGE mouse gene expression spatial database

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EMAGE:532

Bmp5 bone morphogenetic protein 5 ( MGI:88181)
TS11
in situ hybridisation

Data Images


Fig 1A Solloway & Robertson 1999 [PMID:10079236] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.	Fig 1C Solloway & Robertson 1999 [PMID:10079236] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
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Notes:

The middle embryo shown in Panel A was sectioned in the plane shown to produce C. The right hand embryo in A has been spatially mapped in this entry as well as Image C (which was taken from the middle embryo in A) Abbreviations in C: ne - neurectoderm; mes - mesoderm; end - endoderm. In A, the embryos range between 6.5 - 7.5 dpc. The embryo in C is 7dpc.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

3D context movie

wholemount mapping

View mapped 3D expression image

Download individual expression domains:

532_wholemount_strong_3D_2.wlz

532_wholemount_moderate_3D_2.wlz

532_wholemount_notDetected_3D_2.wlz

532_voxel_strong_3D_1.wlz

532_voxel_moderate_3D_1.wlz

532_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo mesoderm

detected

Expression is in the anterior mesoderm Expression is in cranial paraxial mesoderm

heart

detected

regional

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1333123
Entity Detected:	Bmp5, bone morphogenetic protein 5 ( MGI:88181)
Notes:	The probe used in this study by Solloway & Robertson, 1999 [PMID:10079236] is indicated as being originally described by Solloway et al, 1998 [PMID:9664685] ie. as a "~1.0kb fragment of the Bmp5 cDNA comprised of nucleotides -33 to 998 of the pro-region (a gift from David Kingsley, Stanford University)". GenBank entry NM_007555.1 for Bmp5 from Kingsley et al define the pro-region as an 806nt region between nt 103-909 of NM_0007555.1. Exact end-points of probe therefore unclear.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	sectioned wholemount

Procedures

Staining procedure:

alkaline phosphatase + NBT/BCIP

General Information

Authors:	Solloway & Robertson, 1999 [PMID:10079236] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:10079236] Solloway MJ, Robertson EJ 1999 Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development (126):1753-68
	[ doi:10.1002/(SICI)1520-6408(1998)22:4<321::AID-DVG3>3.0.CO;2-8] [ PMID:9664685] Solloway MJ, Dudley AT, Bikoff EK, Lyons KM, Hogan BL, Robertson EJ 1998 Mice lacking Bmp6 function. Dev Genet (22):321-39
Links:	MGI:1333146 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source