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EMAGE:544

Mitf microphthalmia-associated transcription factor ( MGI:104554)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:544
Fig 4J, Martinez-Morales et al, 2001 [PMID:11493524] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: lv, lens vesicle; nr, neural retina; pe, retina pigment epithelium.
Expression Pattern Description
Spatial Annotation:
EMAGE:544EMAGE:544Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
544_voxel_strong_3D_1.wlz
544_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:544_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pigmented retinal epithelium
detected detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:21785
Entity Detected:Mitf, microphthalmia-associated transcription factor ( MGI:104554)
Notes:The Mitf probe used in this study by Martinez-Morales et al, 2001 [PMID:11493524] is indicated as that used by Hodgkinson et al, 1993 [PMID:8343963] ie. "MC-1", a cDNA clone which spans almost the entire ORF including the bHLH domain and a small fragment of the 5' UTR (see Fig 1C therein for map).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:DBA/2 x C57BL/6
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
General Information
Authors:Martinez-Morales et al, 2001 [PMID:11493524] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493524] Martinez-Morales JR, Signore M, Acampora D, Simeone A, Bovolenta P 2001 Otx genes are required for tissue specification in the developing eye. Development (128):2019-30
 [ doi:10.1016/0092-8674(93)90429-T] [ PMID:8343963] Hodgkinson CA, Moore KJ, Nakayama A, Steingrimsson E, Copeland NG, Jenkins NA, Arnheiter H 1993 Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein. Cell (74):395-404
Links:MGI:2137360 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI