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EMAGE:546

Zeb1 zinc finger E-box binding homeobox 1 ( MGI:1344313)
TS21 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:546
Fig 2K of Takagi et al., 1998 [PMID:9389660] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: A and P indicate the direction of anterior-posterior axis; hl-hindlimb bud.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
femur cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 1 metatarsus pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 1 phalanx pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 2 metatarsus pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 2 phalanx pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 3 metatarsus pre-cartilage condensation
detected detected
gradedExpression was present in the perichondrium
hindlimb digit 3 phalanx pre-cartilage condensation
detected detected
gradedExpression was present in the perichondrium
hindlimb digit 4 metatarsus pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 4 phalanx pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 5 metatarsus pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
hindlimb digit 5 phalanx pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
tibia-fibula pre-cartilage condensation
detected detected
regionalExpression was present in the perichondrium
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1197090
Entity Detected:Zeb1, zinc finger E-box binding homeobox 1 ( MGI:1344313)
Sequence:sense strand is shown

>MGI:1197090
GTCGACAGTCAGTAGCGTTTACCAGAACAGTGTTTATTCTGTCCAGGAAGAACCCTTGAACTTGTCTTGT
GCAAAAAAGGAACCACAAAAGGACAGCTGTGTTACAGACTCAGAACCAGTTGTAAATGTAGTCCCACCAA
GTGCCAACCCCATAAACATTGCTATTCCTACAGTCACTGCCCAGTTACCCACAATCGTGGCCATTGCTGA
CCAGAACAGTGTTCCATGTTTAAGAGCACTGGCCGCCAACAAGCAGACTATTCTGATTCCCCAAGTGGCA
TATACATATTCAGCTACTGTGAGCCCTGCCGTGCAGGAGCCGCCAGTGAAGGTGATCCAGCCAAACGGAA
ACCAGGATGAAAGACAAGACACTAGCTCAGAAGGAGTCTCCACTGTGGAGGACCAGAATGACTCTGACTC
CACGCCACCCAAAAAGAAAACTCGGAAGACAGAGAATGGAATGTATGCATGTGACCTGTGTGACAAGATA
TTTCAGAAGAGCAGCTCACTGTTGAGACACAAATATGAGCACACAGGTAAGAGGCCTCACGAGTGTGGAA
TCTGTAGAAAGGCATTTAAACACAAGCATCATTTGATTGAGCACATGCGGCTGCACTCTGGGGAAAAGCC
CTATCAATGTGACAAGTGTGGCAAGCGCTTCTCACACTCCGGCTCCTACTCTCAACACATGAATCACCGC
TACTCCTACTGCAAGAGAGGAGCTGAAGACAGAGATGCTATGGAGCAGGAAGACGCTGGGCC
nt 2200 - nt 2961 of NM_011546.1
Notes:The Zfhx1a (deltaEF1) probe used in this study by Takagi et al., 1998 [PMID:9389660] is described as a "0.8kb SalI-ApaI cDNA fragment".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12.5 dpc
Theiler Stage:TS21
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Takagi et al., 1998 [PMID:9389660] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9389660] Takagi T, Moribe H, Kondoh H, Higashi Y 1998 DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages. Development (125):21-31
Links:MGI:1306682 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI