Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5872

Mef2b myocyte enhancer factor 2B ( MGI:104526)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5872
0608d2.jpgThis embryo was treated with ProteinaseK for either 3min or 30min.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5872Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5872_wholemount__moderate.wlz
5872_wholemount__possible.wlz
5872_wholemount__notDetected.wlz
5872_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5872_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506312
Entity Detected:Mef2b, myocyte enhancer factor 2B ( MGI:104526)
Sequence:sense strand is shown

>MGI:3506312
CTGAGCGTGCTTTGTGACTGCGACATTGCCCTGATCATCTTCAACAGTGCGCAACGCCTCTTCCAGTATG
CGAGCAGCGACATGGACCGGGTGCTGCTCAAATACACCGAGTACAGCGAGCCGCACGAGAGCCGCACCAA
CGCGGATATCCTTCAGACACTTAAGAGGAGGGGCGTGGGCCTTGATGGGCCCGAGCTGGATATGGAGGAG
GGGCCAGAAGGTCCTGGAGAGAAGCTGCTGAGGACACTGGGGGGTGACAGAGGCTCGGCCTCGCCCCGGC
CTCGGATCTATCCCGTGGCCCCTGCAATGTCGGTTTCAGAGCTGTCCTACCGGGTCCCACCAGCCACTCC
AGGCTGTGATCCTGGCGGGCTTGGGGAGGTCCCGTCTGTCCACAGCCGCCCGGCTTACTTTCGACCCCCG
GGCCTGGGACACCCGATCTTCTCTCCAAGCCACCTTGCGAGCAAGACGCCCCCGCCTCTGTACCTGGCGA
CTGATGGGCGGAGGCCAGACCTGCCCCCTGGCCTGGTTGGTGCCCGAGGGGGATTGGGCACCTCGAGAAG
CCTGTACAGCGGCCTGCAGAGTCCAGGTGCCCCAGGCCCTGCTCTGGGTAGCTTTGCCTTCTTACCATCA
GGCTCCACAGATTGCAGCCCGGGAGACGCAGCTCAGGGACCCCTGCAACCCTCACCCTGGCCACCAACGA
GGGACGCTGTAGACCCCGCCCGGCCAGTTGCCCGCAGTCTGTGCAAGGAGGGTCCCCCCAGCCGAGGGGG
CTCCCCTCCGACACCTCCGGTCAGCATCAAGTCTGAGCGA
nt 189 - nt 998 of D50311.1
Notes:The Mef2b probe used in this study by Gray et al., 2004 [PMID:15618518] was generated by PCR with primers CCGGAATTCCTGAGCGTGCTTTGTGACTGC and CCCAAGCTTTCGCTCAGACTTGATGCTGACC.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Gray PA; Fu H; Luo P; Zhao Q; Yu J; Ferrari A; Tenzen T; Yuk DI; Tsung EF; Cai Z; Alberta JA; Cheng LP; Liu Y; Stenman JM; Valerius MT; Billings N; Kim HA; Greenberg ME; McMahon AP; Rowitch DH; Stiles CD; Ma Q, 2004 [PMID:15618518] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Links:MGI:3509007 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE