EMAGE mouse gene expression spatial database

EMAGE:5929

Rapgef1 Rap guanine nucleotide exchange factor (GEF) 1 ( MGI:104580)
TS19 (11.5 dpc)
in situ reporter

Data Images


Figure 3A. [doi:10.1242/dev.00217] Copyright: This image is from Voss et al., Development 130:355-367 (200), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. [PMID:12466202]

Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Ubiquitously expressed.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Rapgef1, Rap guanine nucleotide exchange factor (GEF) 1 ( MGI:104580)
Notes:	Rapgef1 (C3G) expression was assayed in this study by Voss et al., 2003 [PMID:12466202] in embryos heterozygous for a gene trapped allele where the promoter-less reporter construct pGT1.betageo (Skarnes et al., 1995 [PMID:7604039] ) inserted into intron 1 and disrupted the coding region after the first 19 codons. The mutated allele codes for a fusion between the first 19 aa of Rapgef1, beta-galactosidase and neomycin phosphotransferase.
Labelled with:	LacZ

Specimen

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Voss AK; Gruss P; Thomas T, 2003 [PMID:12466202] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00217] [ PMID:12466202] Voss AK, Gruss P, Thomas T 2003 The guanine nucleotide exchange factor C3G is necessary for the formation of focal adhesions and vascular maturation. Development (130):355-67
	[ doi:10.1073/pnas.92.14.6592] [ PMID:7604039] Skarnes WC, Moss JE, Hurtley SM, Beddington RS 1995 Capturing genes encoding membrane and secreted proteins important for mouse development. Proc Natl Acad Sci U S A (92):6592-6
Links:	MGI:3797886 same experiment
	MGI:2445403 allele description
	EMAGE:5364 same reporter
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source