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EMAGE:6212

Hbb-b1 hemoglobin, beta adult major chain ( MGI:96021)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212
euxassay_013139_01 euxassay_013139_02 euxassay_013139_03 euxassay_013139_04 euxassay_013139_05
EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212
euxassay_013139_06 euxassay_013139_07 euxassay_013139_08 euxassay_013139_09 euxassay_013139_10
EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212
euxassay_013139_11 euxassay_013139_12 euxassay_013139_13 euxassay_013139_14 euxassay_013139_15
EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212
euxassay_013139_16 euxassay_013139_17 euxassay_013139_18 euxassay_013139_19 euxassay_013139_20
EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212 EMAGE:6212
euxassay_013139_21 euxassay_013139_22 euxassay_013139_23 euxassay_013139_24 euxassay_013139_25
EMAGE:6212 EMAGE:6212
euxassay_013139_26 euxassay_013139_27

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6212Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6212_wholemount_strong.wlz
6212_wholemount_moderate.wlz
6212_wholemount_weak.wlz
6212_wholemount_possible.wlz
6212_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6212_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
otherstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39401
Entity Detected:Hbb-b1, hemoglobin, beta adult major chain ( MGI:96021)
Sequence:sense strand is shown

>T39401
ACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTG
TGGGGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGA
CCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAA
GGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACC
TTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCA
ATATGATCGTGATTGTGCTGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81128. Forward Primer - name:081128_F_cDNA_Hbb, sequence:ACTCACAACCCCAGAAACAGAC; Reverse Primer - name:081128_N_SP6_cDNA_Hbb, sequence:CCCAGCACAATCACGATCATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6215 same embryo
 EMAGE:6214 same embryo
 EMAGE:6213 same embryo
 EMAGE:6211 same embryo
 EurExpress:euxassay_013139 same experiment
 MGI:4825329 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS