EMAGE mouse gene expression spatial database

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EMAGE:6388

Ccdc88c coiled-coil domain containing 88C ( MGI:1915589)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016252. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016252_01	euxassay_016252_02	euxassay_016252_03	euxassay_016252_04

euxassay_016252_05	euxassay_016252_06	euxassay_016252_07	euxassay_016252_08	euxassay_016252_09

euxassay_016252_10	euxassay_016252_11	euxassay_016252_12	euxassay_016252_13	euxassay_016252_14

euxassay_016252_15	euxassay_016252_16	euxassay_016252_17	euxassay_016252_18	euxassay_016252_19

euxassay_016252_20	euxassay_016252_21	euxassay_016252_22	euxassay_016252_23	euxassay_016252_24

euxassay_016252_25	euxassay_016252_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6388_wholemount_strong.wlz

6388_wholemount_moderate.wlz

6388_wholemount_weak.wlz

6388_wholemount_possible.wlz

6388_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6388_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23 24 25

humerus

weak

regional

weak expression: see section 02 03 25 26

femur

weak

regional

weak expression: see section 03 04 21 22

cerebral cortex mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 15 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 17 18

rest of cerebellum mantle layer

weak

regional

weak expression: see section 12 13 14 16 17 18

pons mantle layer

weak

regional

weak expression: see section 08 09 19 20

midbrain mantle layer

weak

regional

weak expression: see section 09 20 21

ventral grey horn

weak

regional

weak expression: see section 12 13 14 16 17

mandible

moderate

regional

moderate expression: see section 05 06 07 19 20 21 22 weak expression: see section 08 09 17 18

maxilla

weak

regional

weak expression: see section 08 17

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24 25 26

clavicle

moderate

regional

moderate expression: see section 19 weak expression: see section 08 09 10 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45530
Entity Detected:	Ccdc88c, coiled-coil domain containing 88C ( MGI:1915589)
Sequence:	sense strand is shown >T45530 TAGGAAAAGCTGACAGTCCCTCTCCAGGCCAGGGTACCCGAGGCCGGCCACTGGACACAAGACGCTTCTC TCTGGCCCCCCCAAAGGAAGAGAGGCTGGCCCCCCTACAGCAGTCTGCCACAGCCCCAGCCCTTGCTACT GGATGTAGCAGTGGTAGCAACCCCCAGATCCAGCACTTTTCTCCTACAGTGGCTCCTGCGGTCAGGACAA AATCCAAAGTGCCACAACACTCAGGGGAGGTAGCCACCGTAGCTCCTGTGCGTCCAGGGCTCGGCACCTC AGAGGGAGATGGGGGCCCAGGGCATGGTTACAGCGAAGGGCTTCTGACCAAAAGCCCTGGCAGGTCTTCT GACTTGCCTCCCCATGTCAAAAGGGGCCCCGATGACTTCAGTCAAGGGAGCAGTTCAAAGAGCACACCGG CGTCCCCAGAGCCAGGTGGGGACCCGCAGACAGTGTGGTATGAGTACGGCTGCGTGTGACTCGCATCGTG GATCTGCAGCTTGCAAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 225429. Forward Primer - name:225429_F_exon_0610010D24Rik, sequence:TAGGAAAAGCTGACAGTCCCTC; Reverse Primer - name:225429_N_SP6_exon_0610010D24Rik, sequence:ATTTGCAAGCTGCAGATCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6387 same embryo
	EMAGE:6386 same embryo
	EMAGE:6384 same embryo
	EMAGE:6385 same embryo
	EMAGE:6389 same embryo
	EurExpress:euxassay_016252 same experiment
	MGI:4823687 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source