EMAGE mouse gene expression spatial database

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EMAGE:6462

Col10a1 collagen, type X, alpha 1 ( MGI:88445)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016324. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016324_01	euxassay_016324_02	euxassay_016324_03	euxassay_016324_04

euxassay_016324_05	euxassay_016324_06	euxassay_016324_07	euxassay_016324_08	euxassay_016324_09

euxassay_016324_10	euxassay_016324_11	euxassay_016324_12	euxassay_016324_13	euxassay_016324_14

euxassay_016324_15	euxassay_016324_16	euxassay_016324_17	euxassay_016324_18	euxassay_016324_19

euxassay_016324_20	euxassay_016324_21	euxassay_016324_22	euxassay_016324_23	euxassay_016324_24

euxassay_016324_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6462_wholemount_strong.wlz

6462_wholemount_moderate.wlz

6462_wholemount_weak.wlz

6462_wholemount_possible.wlz

6462_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6462_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 03 24 25

hindlimb digit 1 phalanx

weak

regional

weak expression: see section 21

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 21

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 21

hindlimb digit 4 phalanx

weak

regional

weak expression: see section 20

fibula

strong

regional

strong expression: see section 01 02 24 25

tibia

strong

regional

strong expression: see section 01 02 23 24

femur

strong

regional

strong expression: see section 02 03 04 20 21 22

pelvic girdle musculature

strong

regional

strong expression: see section 07 15

axial skeleton

strong

regional

strong expression: see section 08 09 10 14 15 16 17

basioccipital bone

strong

regional

strong expression: see section 11 12 13 14 15

temporal bone

strong

regional

strong expression: see section 06 07 19 20

clavicle

strong

regional

strong expression: see section 07 08 09 17 18 19

scapula

strong

regional

strong expression: see section 01 02 03 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45058
Entity Detected:	Col10a1, collagen, type X, alpha 1 ( MGI:88445)
Sequence:	sense strand is shown >T45058 AGGTATTCCAGGAGAAAAAGGCCACCCAGGGGCTCCAGGAATAGCTGGTCCTCCAGGAGCTCCTGGCTTT GGAAAACAAGGCTTGCCAGGTTTGAGGGGACAAAGGGGACCTGCTGGTCTTCCTGGGGCTCCAGGTGCCA AAGGGGAACGAGGGCCAGCAGGTCATCCTGGAGAACCGGGTCTGCCTGGATCCCCTGGGAATATGGGACC CCAAGGACCTAAAGGAATCCCAGGGAACCATGGCATTCCAGGCGCTAAAGGTGAGATAGGTCTAGTTGGG CCTGCAGGCCCCCCTGGGGCTAGAGGAGCAAGGGGTCCACCTGGGTTAGATGGAAAAACAGGGTATCCTG GGGAGCCAGGTCTCAATGGTCCTAAGGGTAACCCAGGGTTACCAGGACAAAAAGGTGATCCTGGAGTGGG AGGAACCCCTGGTCTTCGAGGTCCTGTTGGCCCTGTAGGAGCTAAAGGAGTGCCTGGACACAATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 212507. Forward Primer - name:212507_F_exon_Col10a1, sequence:AGGTATTCCAGGAGAAAAAGGC; Reverse Primer - name:212507_N_SP6_exon_Col10a1, sequence:CATTGTGTCCAGGCACTCCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6459 same embryo
	EMAGE:6461 same embryo
	EMAGE:6458 same embryo
	EMAGE:6460 same embryo
	EurExpress:euxassay_016324 same experiment
	MGI:4823973 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source