EMAGE mouse gene expression spatial database

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EMAGE:6474

Mir210 microRNA 210 ( MGI:2676886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019072_01	euxassay_019072_02	euxassay_019072_03	euxassay_019072_04

euxassay_019072_05	euxassay_019072_06	euxassay_019072_07	euxassay_019072_08	euxassay_019072_09

euxassay_019072_10	euxassay_019072_11	euxassay_019072_12	euxassay_019072_13	euxassay_019072_14

euxassay_019072_15	euxassay_019072_16	euxassay_019072_17	euxassay_019072_18	euxassay_019072_19

euxassay_019072_20	euxassay_019072_21	euxassay_019072_22	euxassay_019072_23	euxassay_019072_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6474_wholemount_strong.wlz

6474_wholemount_moderate.wlz

6474_wholemount_weak.wlz

6474_wholemount_possible.wlz

6474_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6474_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 03

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 03 04 05

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 04 05 24

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 09 10 13 14 15 16 17 18 weak expression: see section 08

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 12 13 14 15 16 weak expression: see section 08

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 09 10 11 13 14 15 16 17 18 19 20 21 weak expression: see section 04 05 06 07 08

pons mantle layer

moderate

regional

moderate expression: see section 09 10 12 13 14 15 16 17 18 19 weak expression: see section 05 06 07 08

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 05

facial vii ganglion

moderate

single cell

moderate expression: see section 04 20

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

trigeminal v nerve

moderate

single cell

moderate expression: see section 08 16

spinal cord mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

dorsal root ganglion

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 12 13 14 15 16 17 18

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70335
Entity Detected:	Mir210, microRNA 210 ( MGI:2676886)
Sequence:	sense strand is shown >T70335 CTGTGCGTGTGACAGCGGCTGA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-210 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6477 same embryo
	EMAGE:6475 same embryo
	EMAGE:6476 same embryo
	EurExpress:euxassay_019072 same experiment
	MGI:4826247 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source