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EMAGE:6525

Mir291a microRNA 291a ( MGI:3711324)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525
"Pseudo-wholemount" of euxassay_019064. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019064_01 euxassay_019064_02 euxassay_019064_03 euxassay_019064_04
EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525
euxassay_019064_05 euxassay_019064_06 euxassay_019064_07 euxassay_019064_08 euxassay_019064_09
EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525
euxassay_019064_10 euxassay_019064_11 euxassay_019064_12 euxassay_019064_13 euxassay_019064_14
EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525
euxassay_019064_15 euxassay_019064_16 euxassay_019064_17 euxassay_019064_18 euxassay_019064_19
EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525 EMAGE:6525
euxassay_019064_20 euxassay_019064_21 euxassay_019064_22 euxassay_019064_23 euxassay_019064_24
EMAGE:6525 EMAGE:6525
euxassay_019064_25 euxassay_019064_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6525Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6525_wholemount_strong.wlz
6525_wholemount_moderate.wlz
6525_wholemount_weak.wlz
6525_wholemount_possible.wlz
6525_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6525_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
humerus
moderate moderate
regionalmoderate expression: see section 01 23 24 25 26 weak expression: see section 02 03 04 21 22
femur
moderate moderate
regionalmoderate expression: see section 01 02 03 24 25 weak expression: see section 04 06 22 23
thymus primordium
strong strong
regionalstrong expression: see section 10 11 13 14 moderate expression: see section 12
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 05 06 07 08 09 16 17 18 20
pancreas
weak weak
regionalweak expression: see section 09 10 11 12
thyroid gland
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 14 15
dermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
vibrissa
weak weak
regionalweak expression: see section 05 06 07 21
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 11 12
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 11 12
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23 24 weak expression: see section 25
cerebral cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 18 19
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 16 17
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 11 12 14 17 weak expression: see section 09 10
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14
ventral grey horn
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 08 15
cervical ganglion
weak weak
regionalweak expression: see section 07 08 16
thoracic ganglion
weak weak
regionalweak expression: see section 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 08 09 10 11 14 15 16
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 23 24 25 26 weak expression: see section 02
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 22 23 24 25 26
naris
weak weak
regionalweak expression: see section 13 14 16
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
vomeronasal organ
weak weak
regionalweak expression: see section 12
stomach
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 02 03 04 05 06
midgut
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 weak expression: see section 08 09 10 11 12
meckel's cartilage
strong strong
regionalstrong expression: see section 05 06 07 08 14 15 16 17 18 19 20 21 moderate expression: see section 09 10 11 12 22 weak expression: see section 13
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24 25 26 moderate expression: see section 08 20
bladder
weak weak
regionalweak expression: see section 13 14 15 16
metanephros
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 05 06 07 08 16 17 18 19 20
testis
moderate moderate
regionalmoderate expression: see section 05 21 weak expression: see section 06 07 19 20
urethra of male
weak weak
regionalweak expression: see section 14 15
lung
moderate moderate
regionalmoderate expression: see section 09 10 17 weak expression: see section 02 03 04 05 06 07 08 11 12 13 14 15 16 18 19 20 21 22
axial skeleton
moderate moderate
regionalmoderate expression: see section 11 12 13 14
scapula
moderate moderate
regionalmoderate expression: see section 01 02 22 weak expression: see section 23
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70363
Entity Detected:Mir291a, microRNA 291a ( MGI:3711324)
Sequence:sense strand is shown

>T70363
CATCAAAGTGGAGGCCCTCTCT
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-291a-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6524 same embryo
 EMAGE:6526 same embryo
 EMAGE:6528 same embryo
 EMAGE:6527 same embryo
 EMAGE:6523 same embryo
 EurExpress:euxassay_019064 same experiment
 MGI:4826252 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS