EMAGE mouse gene expression spatial database

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EMAGE:6605

Mir615 microRNA 615 ( MGI:3629927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019042. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019042_01	euxassay_019042_02	euxassay_019042_03	euxassay_019042_04

euxassay_019042_05	euxassay_019042_06	euxassay_019042_07	euxassay_019042_08	euxassay_019042_09

euxassay_019042_10	euxassay_019042_11	euxassay_019042_12	euxassay_019042_13	euxassay_019042_14

euxassay_019042_15	euxassay_019042_16	euxassay_019042_17	euxassay_019042_18	euxassay_019042_19

euxassay_019042_20	euxassay_019042_21	euxassay_019042_22	euxassay_019042_23	euxassay_019042_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6605_wholemount_strong.wlz

6605_wholemount_moderate.wlz

6605_wholemount_weak.wlz

6605_wholemount_possible.wlz

6605_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6605_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 07 08 10 15 18 20 21

humerus

weak

regional

weak expression: see section 01 02 03 23 24

fibula

weak

regional

weak expression: see section 21 22 23

tibia

weak

regional

weak expression: see section 21 22

femur

weak

regional

weak expression: see section 01 02 03 04 18 19 21 22

otic capsule

weak

regional

weak expression: see section 08 09 16 17

meckel's cartilage

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 18 19 20

axial skeleton

weak

regional

weak expression: see section 09 10 11 12 15 16 18 19

basisphenoid bone

weak

regional

weak expression: see section 10 11 12 13 14 15 16

exoccipital bone

weak

regional

weak expression: see section 05 06 07 21 22 23 24

temporal bone

weak

regional

weak expression: see section 03 04 05 06 20 21 22 23 24

orbito-sphenoid

weak

regional

weak expression: see section 05 06 07 10 11 12 13 14 15 17 18 19 21 22 24

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 04 23 24

pelvic girdle skeleton

weak

regional

weak expression: see section 07 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70102
Entity Detected:	Mir615, microRNA 615 ( MGI:3629927)
Sequence:	sense strand is shown >T70102 TCCGAGCCTGGGTCTCCCTCTT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-615-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6606 same embryo
	EMAGE:6604 same embryo
	EMAGE:6607 same embryo
	EurExpress:euxassay_019042 same experiment
	MGI:4826336 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source