EMAGE mouse gene expression spatial database

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EMAGE:6609

Mir207 microRNA 207 ( MGI:2676882)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019033. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019033_01	euxassay_019033_02	euxassay_019033_03	euxassay_019033_04

euxassay_019033_05	euxassay_019033_06	euxassay_019033_07	euxassay_019033_08	euxassay_019033_09

euxassay_019033_10	euxassay_019033_11	euxassay_019033_12	euxassay_019033_13	euxassay_019033_14

euxassay_019033_15	euxassay_019033_16	euxassay_019033_17	euxassay_019033_18	euxassay_019033_19

euxassay_019033_20	euxassay_019033_21	euxassay_019033_22	euxassay_019033_23	euxassay_019033_24

euxassay_019033_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6609_wholemount_strong.wlz

6609_wholemount_moderate.wlz

6609_wholemount_weak.wlz

6609_wholemount_possible.wlz

6609_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6609_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 13 14 17 weak expression: see section 11 12 15 16

cerebral cortex mantle layer

moderate

single cell

moderate expression: see section 02 03 04 05 13 17

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 13 14 17 18 19 21 22 23 24 weak expression: see section 11

olfactory cortex mantle layer

moderate

single cell

moderate expression: see section 09 10 14 17 weak expression: see section 11 15 16

medulla oblongata alar plate mantle layer

moderate

single cell

moderate expression: see section 08 09 10 17 18

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 14 17 18

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 13 14 17 18 19 20 21 weak expression: see section 15 16

pons mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 17 18 19

midbrain mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 13 14 16 17 18 weak expression: see section 11 12 15

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 21 22

ventral grey horn

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 10 18 19 weak expression: see section 11 12 13 14 17

lens

weak

regional

weak expression: see section 01 25

retina

weak

regional

weak expression: see section 01

neural retina

weak

regional

weak expression: see section 02 03 25

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70329
Entity Detected:	Mir207, microRNA 207 ( MGI:2676882)
Sequence:	sense strand is shown >T70329 GCTTCTCCTGGCTCTCCTCCCTC
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-207 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6608 same embryo
	EurExpress:euxassay_019033 same experiment
	MGI:4826246 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source