EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6621

Mir292 microRNA 292 ( MGI:3711325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019067. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019067_01	euxassay_019067_02	euxassay_019067_03	euxassay_019067_04

euxassay_019067_05	euxassay_019067_06	euxassay_019067_07	euxassay_019067_08	euxassay_019067_09

euxassay_019067_10	euxassay_019067_11	euxassay_019067_12	euxassay_019067_13	euxassay_019067_14

euxassay_019067_15	euxassay_019067_16	euxassay_019067_17	euxassay_019067_18	euxassay_019067_19

euxassay_019067_20	euxassay_019067_21	euxassay_019067_22	euxassay_019067_23	euxassay_019067_24

euxassay_019067_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6621_wholemount_strong.wlz

6621_wholemount_moderate.wlz

6621_wholemount_weak.wlz

6621_wholemount_possible.wlz

6621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 10 17 18 21 22 23 24 25 weak expression: see section 03 04 05 06 07 08 09 11 16 19 20

humerus

moderate

regional

moderate expression: see section 02 24 25 weak expression: see section 01 03 04 05 23

hindlimb digit 1 metatarsal

weak

regional

weak expression: see section 24

hindlimb digit 1 phalanx

weak

regional

weak expression: see section 24

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 05 24

hindlimb digit 2 phalanx

weak

regional

weak expression: see section 05 24

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 05 24

hindlimb digit 3 phalanx

weak

regional

weak expression: see section 05 06 23 24

hindlimb digit 4 phalanx

weak

regional

weak expression: see section 06 23

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 23

fibula

weak

regional

weak expression: see section 01 02 03

tibia

weak

regional

weak expression: see section 02 03

femur

moderate

regional

moderate expression: see section 24 25 weak expression: see section 02 04 05 06 07 08 09 21 22 23

otic capsule

weak

regional

weak expression: see section 08 09 10 17 18

hyoid

weak

regional

weak expression: see section 11 12 13 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 10 11 12 17 18 19 20 21 22 23 24 weak expression: see section 03 04 05 06 07 08 09 15 16

axial skeleton

moderate

regional

moderate expression: see section 09 10 17 18 weak expression: see section 08 11 12 13 14 15 16

basisphenoid bone

moderate

regional

moderate expression: see section 09 17 18 weak expression: see section 10 11 12 13 14 15 16

exoccipital bone

moderate

regional

moderate expression: see section 21 weak expression: see section 04 05 06 19 20

temporal bone

moderate

regional

moderate expression: see section 21 weak expression: see section 04 05 06 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 09 21 22 weak expression: see section 07 08 11 12 13 14 15 16 19 20

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

moderate

regional

moderate expression: see section 23 24 25 weak expression: see section 02 03 04

pelvic girdle skeleton

moderate

regional

moderate expression: see section 18 weak expression: see section 11 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70367
Entity Detected:	Mir292, microRNA 292 ( MGI:3711325)
Sequence:	sense strand is shown >T70367 ACTCAAACTGGGGGCTCTTTTG
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-292-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6622 same embryo
	EMAGE:6623 same embryo
	EurExpress:euxassay_019067 same experiment
	MGI:4826254 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source