EMAGE mouse gene expression spatial database

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EMAGE:6677

Krt19 keratin 19 ( MGI:96693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007360. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007360_01	euxassay_007360_02	euxassay_007360_03	euxassay_007360_04

euxassay_007360_05	euxassay_007360_06	euxassay_007360_07	euxassay_007360_08	euxassay_007360_09

euxassay_007360_10	euxassay_007360_11	euxassay_007360_12	euxassay_007360_13	euxassay_007360_14

euxassay_007360_15	euxassay_007360_16	euxassay_007360_17	euxassay_007360_18	euxassay_007360_19

euxassay_007360_20	euxassay_007360_21	euxassay_007360_22	euxassay_007360_23	euxassay_007360_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6677_wholemount_strong.wlz

6677_wholemount_moderate.wlz

6677_wholemount_weak.wlz

6677_wholemount_possible.wlz

6677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

peritoneal component

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 15 16 17 18 19 20 21 22 23 24

pleural cavity

moderate

regional

moderate expression: see section 03 10 14

pleura

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

spotted

moderate expression: see section 11 12 14 15 weak expression: see section 13

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16 17 18 19

skin

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19 20 21 22

inner ear

moderate

regional

moderate expression: see section 05 06 07 08 09 16 17 18 19 20 21

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22 23 24

eyelid

strong

regional

strong expression: see section 01 02 03 21 22 23 24

anterior naris

strong

regional

strong expression: see section 09 10 11 13 14 moderate expression: see section 12

external naris

strong

regional

strong expression: see section 09

nasal cavity epithelium

strong

regional

strong expression: see section 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 14 15 16 17

naso-lacrimal duct

strong

regional

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

pharynx

strong

regional

strong expression: see section 11 12 13 14 15

esophagus

strong

regional

strong expression: see section 12

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

rectum

strong

regional

strong expression: see section 10 11

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 09 10 11 14 15

lower jaw molar

strong

regional

strong expression: see section 06 07 17 18

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 09 10 13 14

upper jaw molar

strong

regional

strong expression: see section 06 07 18

bladder

strong

regional

strong expression: see section 10 11 12

urogenital mesentery

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 17 18 19 20 21 22

kidney calyx

strong

regional

strong expression: see section 06 07 16 17 moderate expression: see section 18

ureter

strong

regional

strong expression: see section 08 09 13 14 15

urethra of male

strong

regional

strong expression: see section 10 11

larynx

strong

regional

strong expression: see section 12 13 14

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

trachea

strong

regional

strong expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3049
Entity Detected:	Krt19, keratin 19 ( MGI:96693)
Sequence:	sense strand is shown >T3049 TGGCCTCGAGCCAGATTCGTCGACTCGCCAGGTGGGTGGCCAGGTCAGTGTGGAGGTGGATTCCACTCCC GGTGTCGACCTAGCCAAGATCCTGAGTGAGATGAGAAGTCAGTATGAGATCATGGCCGAGAAGAACCGGA AGGATGCTGAAGCCACCTACCTTGCTCGGATTGAGGAGCTGAACACCCAGGTCGCCGTCCACTCTGAGCA GATCCAGATAAGCAAGACCGAAGTCACGGACCTTCGACGGACCCTCCAGGGCCTTGAGATTGAGCTGCAG TCCCAGCTCAGCATGAAAGCTGCCCTGGAAGGCACGCTGGCAGAGACGGAGGCCCGTTATGGAGTCCAGC TGTCACAGATCCAGAGCGTGATCAGCGGTTTTGAAGCCCAGCTGAGCGACGTGCGTGCCGACATAGAGCG CCAGAACCAGGAGTATAAGCAGCTCATGGACATCAAGTCCAGGCTGGAGCAGGAGATCGCCACCTACCGC AGCCTGCTGGAGGGCCAGGAAGCCCACTACAACAATCTGCCCACCCCCAAGGCCATCTGAGCTACCA
Notes:	The probe template was PCR amplified from IMAGE:1546202 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546202 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6676 same embryo
	EMAGE:6674 same embryo
	EMAGE:6675 same embryo
	EMAGE:6673 same embryo
	EMAGE:6672 same embryo
	EurExpress:euxassay_007360 same experiment
	MGI:4825831 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source