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EMAGE:669

Myf5 myogenic factor 5 ( MGI:97252)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:669 EMAGE:669 EMAGE:669 EMAGE:669
Fig 1E Borycki et al, 1999 [PMID:10457014] .Copyright: This image are from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1F Borycki et al, 1999 [PMID:10457014] .Copyright: This image are from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 2C Borycki et al, 1999 [PMID:10457014] .Copyright: This image are from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 2I Borycki et al, 1999 [PMID:10457014] .Copyright: This image are from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
E is a higher power view of F. C and I are section taken through interlimb region and tail respectively. Image annotations: In E, the yellow arrow = region of Myf5 expression in the epaxial somite that is lost in Shh null embryos; Black arrowhead = hypaxial domain of somite. In C and I, nt = neural tube; Black arrowheads = Myf5 expression in the epaxial and hypaxial domain of newly formed somites of wild-type embryos. Image Annotations: E and F: yellow arrow - epaxial somite domain, black arrowhead- hypaxial domain, black arrows- limbs, hypoglossal chords and branchial arches, red arrows - expression domain in the head that is missing in Shh(tm1Chg)/Shh(tm1Chg) mutants. C and I: nt - neural tube, black arrowhead - epaxial and hypaxial domains of the somite.
Expression Pattern Description
Spatial Annotation:
EMAGE:669Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
669_wholemount_strong_3D_1.wlz
669_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:669_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is in the ventrolateral domain where hypaxial muscle progenitor cells arise (Figs 1e, 1f, 2c) and expression is in the epaxial and hypaxial domains of the newly formed somites (Fig 2i)
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1342952
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Notes:The Myf5 probe used in this study by Borycki et al, 1999 [PMID:10457014] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
General Information
Authors:Borycki et al, 1999 [PMID:10457014] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10457014] Borycki AG, Brunk B, Tajbakhsh S, Buckingham M, Chiang C, Emerson CP 1999 Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development (126):4053-63
Links:MGI:1343824 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI