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EMAGE:67

Wnt1 wingless-related MMTV integration site 1 ( MGI:98953)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:67
Figure 3G of Suda et al., 2001 [PMID:11493561] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:67Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
67_wholemount_strong_3D_1.wlz
67_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:67_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
regionalExpression in the dorsal midline over the caudal diencephalon and mesencephalon, and in a characteristic lateral stripe at the isthmus.
future midbrain
detected detected
regionalExpression in the dorsal midline over the caudal diencephalon and mesencephalon, and in a characteristic lateral stripe at the isthmus.
future hindbrain
detected detected
regionalAnterior terminus of expression in roof of spinal cord at rhombomere 3 (r3).
future spinal cord
detected detected
regionalAnterior terminus of expression in roof of spinal cord at rhombomere 3 (r3).
rhombomere 03
detected detected
regionalAnterior terminus of expression in roof of spinal cord at rhombomere 3 (r3).
rhombomere 01
not detected not detected
Caudal to isthmus, no expression in the anterior metencephalon, rhombomeres 1 and 2 (r1 and r2).
rhombomere 02
not detected not detected
Caudal to isthmus, no expression in the anterior metencephalon, rhombomeres 1 and 2 (r1 and r2).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328384
Entity Detected:Wnt1, wingless-related MMTV integration site 1 ( MGI:98953)
Sequence:sense strand is shown

>MGI:1328384
TCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTCCGGGGAGAAGGGGCGGGACCTACGCTTCCT
CATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTGTTCTCTGAGATGCGCCAAGAGTGCAAATGC
CACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGATGCGGCTGCCCACGCTGCGCGCTGTGGGCG
ACGTGCTGCGCGACCGCTTCGACGGCGCCTCCCGCGTCCTTTACGGCAACCGAGGCAGCAACCGCGCCTC
GCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCGCACAAGCCTCCCTCCCCTCACGACCTCGTC
TACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCCGCCTGGGCACAGCTGGCACAGCTGGACGAG
CTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCTGCTGTGCTGTGGCCGAGGCCACCGCACGCG
CACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCACTGGTGCTGCCACGTCAGCTGCCGCAACTGC
ACGCACACGCGCGTTCTGCACGAGTGTCTATGAGGTGCCGCGCCTCCGGGAACGGGAACGCTCTCTTCCA
GTTCTCAGACACACTCGCTGGTCCTGATGTTTGCCCACCCTACCGCGTCCAGCCACAGTCCCAGGGTTCA
TAGCGATCCATCTCTCCCACCTCCTACCTGGGGACTCCTGAAACCACTTGCCTGAGTCGGCTCGAACCCT
TTTGCCATCCTGAGGGCCCTGACCCAGCCTACCTCCCTCCCTCTTTGAGGGAGACTCCTTTTGCACTGCC
CCCCAATTTGGCCAGAGGGTGAGAGAAAGATTCTTCTTCTGGGGTGGGGGTGGGGAGGTCAACTCTTGAA
GGTGTTGCGGTTCCTGATGTATTTTGCGCTGTGACCTCTTTGGGTATTATCACCTTTCCTTGTCTCTCGG
GTCCCTATAGGTCCCTTGAGTTCTCTAACCAGCACCTCTGGGCTTCAAGGCCTTTCCCCTCCCACCTGTA
GCTGAAGAGTTTCCGAGTTGAAAGGGCACGGAAAGCTAAGTGGGAAAGGAGGTTGCTGGACCCAGCAGCA
AAACCCTACATTCTCCTTGTCTCTGCCTCGGAGCCATTGAACAGCTGTGAACCATGCCTCCCTCAGCCTC
CTCCCACCCCTTCCTGTCCTGCCTCCTCATCACTGTGTAAATAATTTGCACCGAAATGTGGCCGCAGAGC
CA
nt 705 - nt 1966 of M11943.1
Notes:Probe used in this study by Suda et al., 2001 [PMID:11493561] is indicated as being originally described by McMahon & Bradley, 1990 [PMID:2205396] ie. a "1263 bp ClaI-MluI (Fig. 2, positions 704-1966 in Fung et al., 1985 [PMID:3018519] ) Wnt-1 cDNA probe".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:undefined
General Information
Authors:Suda et al., 2001 [PMID:11493561] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493561] Suda Y, Hossain ZM, Kobayashi C, Hatano O, Yoshida M, Matsuo I, Aizawa S 2001 Emx2 directs the development of diencephalon in cooperation with Otx2. Development (128):2433-50
 [ PMID:3018519] Fung YK, Shackleford GM, Brown AM, Sanders GS, Varmus HE 1985 Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol Cell Biol (5):3337-44
 [ doi:10.1016/0092-8674(90)90385-R] [ PMID:2205396] McMahon AP, Bradley A 1990 The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell (62):1073-85
Links:MGI:2148283 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI