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EMAGE:6769

Pif1 PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) ( MGI:2143057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769
"Pseudo-wholemount" of euxassay_007343. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007343_01 euxassay_007343_02 euxassay_007343_03 euxassay_007343_04
EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769
euxassay_007343_05 euxassay_007343_06 euxassay_007343_07 euxassay_007343_08 euxassay_007343_09
EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769
euxassay_007343_10 euxassay_007343_11 euxassay_007343_12 euxassay_007343_13 euxassay_007343_14
EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769
euxassay_007343_15 euxassay_007343_16 euxassay_007343_17 euxassay_007343_18 euxassay_007343_19
EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769 EMAGE:6769
euxassay_007343_20 euxassay_007343_21 euxassay_007343_22 euxassay_007343_23 euxassay_007343_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6769Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6769_wholemount_strong.wlz
6769_wholemount_moderate.wlz
6769_wholemount_weak.wlz
6769_wholemount_possible.wlz
6769_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6769_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
body cavity or lining
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
limb
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
mesenchyme
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vertebral axis musculature
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
gland
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
visceral organ system
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
integumental system
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
diencephalon lateral wall ventricular layer
moderate moderate
spottedmoderate expression: see section 11 12 13 14
telencephalon ventricular layer
moderate moderate
spottedmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
medulla oblongata alar plate ventricular layer
moderate moderate
spottedmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20
medulla oblongata basal plate ventricular layer
moderate moderate
spottedmoderate expression: see section 12 13 15 16
rest of cerebellum ventricular layer
moderate moderate
spottedmoderate expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23
pons ventricular layer
moderate moderate
spottedmoderate expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23
midbrain ventricular layer
moderate moderate
spottedmoderate expression: see section 10 11 12 13 14 15 16 17 18 19
spinal cord ventricular layer
moderate moderate
spottedmoderate expression: see section 13 14
sensory organ system
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
cardiovascular system
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
skeleton
moderate moderate
spottedmoderate expression: see section 05
tail
moderate moderate
spottedmoderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1817
Entity Detected:Pif1, PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) ( MGI:2143057)
Sequence:sense strand is shown

>T1817
TGGCCTCGAGGCCAGAATTCGGAACGAGGGCTGAAAGAGAAGACAAACTTTTAGCTTTTTTTCCTGGGTC
AAGGCCCTAGGAATTAACTGGGGAGAGGCCTGTGTTTCTTCCCTTATTCAGCCTCTGGTAGGGTTAAGGG
ACACAGTTTCCCATCTACTTAACTAGCATTGCCTCAGTTTCACCTATTTCCCCGGGGAAATGACTTCCAG
GGTTCAAAGCTAGAAATGGTGATGGTTACCAGAGGACAAAGCTCTCTACCAAGGGTGGAACACACAGCCA
CAGAGTTCTTTGCAGGCTGGAGAGGCAGTGCGGGCAGGGGCTGCATTCAGCAGCAGCAGCAGTAGGAGCA
GCCTGTCTTATTACACCGCATGTATTTATTTTGTGTGCTTGTGCACGCACAGCATATTGTACATGTGAAG
GTCAGAGGACAACTCGAGGAAGTTGGTTTTCTCTTTCCCCAAGTGTGTTCTGGGGGTTAAATTCAGGTCA
CAGGGCTTGGTAGCAGGCACTTATACCCGATGAGCAATCTTGCTACCAGGGTCGGTTCTAATTTTCTTTG
TGTTATTATAA
Notes:The probe template was PCR amplified from IMAGE:576860 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:576860 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6768 same embryo
 EMAGE:6770 same embryo
 EMAGE:6771 same embryo
 EurExpress:euxassay_007343 same experiment
 MGI:4827200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS