EMAGE mouse gene expression spatial database

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EMAGE:6921

Trp63 transformation related protein 63 ( MGI:1330810)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002524. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002524_01	euxassay_002524_02	euxassay_002524_03	euxassay_002524_04

euxassay_002524_05	euxassay_002524_06	euxassay_002524_07	euxassay_002524_08	euxassay_002524_09

euxassay_002524_10	euxassay_002524_11	euxassay_002524_12	euxassay_002524_13	euxassay_002524_14

euxassay_002524_15	euxassay_002524_16	euxassay_002524_17	euxassay_002524_18	euxassay_002524_19

euxassay_002524_20	euxassay_002524_21	euxassay_002524_22	euxassay_002524_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6921_wholemount_strong.wlz

6921_wholemount_moderate.wlz

6921_wholemount_weak.wlz

6921_wholemount_possible.wlz

6921_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6921_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 12 13 weak expression: see section 11

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 14 15 16 17 18

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 03 04 05 06 07 12 13 19 20 21 22

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 20 21 22 23

naris

moderate

regional

moderate expression: see section 10 11 12 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15

esophagus

strong

regional

strong expression: see section 10 11

stomach

strong

regional

strong expression: see section 01 02 03 04

lower jaw incisor

strong

regional

strong expression: see section 09 10 11 13 14 15

lower jaw molar

strong

regional

strong expression: see section 06 18

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 10 11 14 15

upper jaw molar

strong

regional

strong expression: see section 06 18

urethra of male

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2041
Entity Detected:	Trp63, transformation related protein 63 ( MGI:1330810)
Sequence:	sense strand is shown >T2041 TCGAGGNCAGATTCGGCACGAGGGCAAATGAGTCCTTAATTTCAACATTTTGTTGTGTTTAAATAATGAT AAGCATCATTAACTTCTGTAACAAACTCACAGCTTTACAAATTCAATGGGTGGAGAAGAAAGCTGTGTCT TAGCCATGTTAGGAAGACAAATGGCTTCCTGTGTGTTGTAAGTATTTGGGCTGTTTCAGCAGTGTTGGTG TGGCACAGGGGACTCTGTGGCATTTCAGCACTATTTAGGTGGCACTAGGGACTCTGAAATTCCTGTACTG TATCTGATGATTTTGGCATTAGCCATAGGTAGGCACAGTTTGTCTCCTCACACCAGTGTTTAGTGTGTGA ATAGCCAGAGCTGTGGGGAAGAACACAGAGAACAGACATCTGCTGGATGCCTCTCAGTGGAGAATGGGAT TCCTTCACTTGGTGGTGAAGCAGATAGGATAGAAAGCAGGATTCTCTTTGTTAATCCAGTTAGCTTTTGT TTTCTTGATATCCCCCCTGAATACGTTGAGTATGAGAGATATGTGGGTTTTTT
Notes:	The probe template was PCR amplified from IMAGE:762745 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:762745 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6917 same embryo
	EMAGE:6918 same embryo
	EMAGE:6920 same embryo
	EMAGE:6916 same embryo
	EMAGE:6919 same embryo
	EMAGE:6922 same embryo
	EurExpress:euxassay_002524 same experiment
	MGI:4828935 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source