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EMAGE:700

T brachyury ( MGI:98472)
TS10 (7.75 dpc)
in situ hybridisation

Data Images
EMAGE:700
Figure 4D of Smith et al., 1992 [PMID:1295727] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ee - embryonic ectoderm; m - mesoderm; ps - primitive streak.
Expression Pattern Description
Spatial Annotation:
EMAGE:700EMAGE:700Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
700_voxel_strong_3D_1.wlz
700_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:700_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo mesoderm
strong strong
regionalHigh levels of expression in mesoderm adjacent (medial) to the primitive streak, but not in more lateral mesoderm
primitive streak
strong strong
High levels of expression in the primitive streak
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2449092
Entity Detected:T, brachyury ( MGI:98472)
Sequence:sense strand is shown

>MGI:2449092
GGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGG
AGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAA
GAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAG
GGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGC
TAACTAACGAGATGATTGTGACCAAGAACGGCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGG
CCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATAT
GTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAG
ACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAA
GCTCAATGGAGGGGGACAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGA
GTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCT
ACCAGAATGAGGAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAA
AGAAAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGG
GGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGC
TCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCC
CAGCCCCTATGCTCATCGGAACAGCTCTCCAACCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTG
CAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACG
CCAGCCCACCTACTGGCTCTAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGG
CTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACA
CCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTA
CAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGT
GTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGA
TCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACA
ACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCT
ACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGA
CTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGA
CTTTGAAATCCTGG
nt 1 - nt 1764 of X51683.1
Notes:The T probe used in this study by Smith et al., 1992 [PMID:1295727] is indicated as that previously described by Herrmann, 1991 [PMID:1821859] as "the sequence from base 1 to base 1764 of the cDNA for the T gene, pme75 (Herrmann et al., 1990 [PMID:2154694] )."
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:C57BL/6
Age:7.75 dpc
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Smith et al., 1992 [PMID:1295727] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1295727] Smith DE, Franco del Amo F, Gridley T 1992 Isolation of Sna, a mouse gene homologous to the Drosophila genes snail and escargot: its expression pattern suggests multiple roles during postimplantation development. Development (116):1033-9
 [ PMID:1821859] Herrmann BG 1991 Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development (113):913-7
 [ doi:10.1038/343617a0] [ PMID:2154694] Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H 1990 Cloning of the T gene required in mesoderm formation in the mouse. Nature (343):617-22
Links:MGI:2153808 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI