EMAGE mouse gene expression spatial database

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EMAGE:7061

Hdgf hepatoma-derived growth factor ( MGI:1194494)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002405. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002405_01	euxassay_002405_02	euxassay_002405_03	euxassay_002405_04

euxassay_002405_05	euxassay_002405_06	euxassay_002405_07	euxassay_002405_08	euxassay_002405_09

euxassay_002405_10	euxassay_002405_11	euxassay_002405_12	euxassay_002405_13	euxassay_002405_14

euxassay_002405_15	euxassay_002405_16	euxassay_002405_17	euxassay_002405_18	euxassay_002405_19

euxassay_002405_20	euxassay_002405_21	euxassay_002405_22	euxassay_002405_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7061_wholemount_strong.wlz

7061_wholemount_moderate.wlz

7061_wholemount_weak.wlz

7061_wholemount_possible.wlz

7061_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7061_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 09 moderate expression: see section 10 11 12 13 14

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 16 17 18 moderate expression: see section 19

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

naris

moderate

regional

moderate expression: see section 12 13

nasal cavity epithelium

moderate

regional

moderate expression: see section 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16 17

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 18 19

oral epithelium

moderate

regional

moderate expression: see section 06 10 11 12 13 14 15 16 17 18 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 18 19

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 21 22 weak expression: see section 01 02 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2974
Entity Detected:	Hdgf, hepatoma-derived growth factor ( MGI:1194494)
Sequence:	sense strand is shown >T2974 TGNNCTCGAGGNCAGATTCGGACGAGGTTTTTTTTTAGGAAATGTTTTTAATAAAAGAAAATTACCAAAA AAGAAAAAAAAAAACCCAACCCACAAAAGAAGAAAAAAAGGAAAAAAGTTGCCCCGCCCCTTTGTGTGTT CCTCTCTCTGCTCCTTTCTCCCCACTGCTGACCCGTTTTTTGAGGTGCGCTAGGAGGGAGGCTCGCCTCT GACCGGGTTGGCTTTCTCTTTGGACATGGGGCTTTGATGTTCTTCCAGAGTTGGTCTCTGGTGTTTTCTC ACATCCAGTTTGTAGCCCACTAAGAATACAGGGAAAAGTGTCCTCCAGCCTCTTCTAGTGGTTTCTTTAC ATGCCCCTCTTCCTTGTCTTTTACTCCCATCTCTCCTCTGGGCTCTGAAGAAAAATTGCTGACTGTAGCT TTGGAAGTGTAGCTCTGAGAATAATAGAAAATTTGAGTTCTAGGAAAATAAAGCCGTTGATTACTATCCT GAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1532916 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532916 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7065 same embryo
	EMAGE:7062 same embryo
	EMAGE:7064 same embryo
	EMAGE:7063 same embryo
	EMAGE:7060 same embryo
	EurExpress:euxassay_002405 same experiment
	MGI:4825346 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source