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EMAGE:714

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:714
Fig 5A Kanzler et al, 2000 [PMID:10662648] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: V; VII/VIII; IX, X = fifth - tenth cranial ganglia/nerves.
Expression Pattern Description
Spatial Annotation:
EMAGE:714Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
714_wholemount_strong_3D_1.wlz
714_wholemount_moderate_3D_1.wlz
714_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:714_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
central nervous system ganglion
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:28469
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The Nef3 (NF-M) probe used in this study by Kanzler et al, 2000 [PMID:10662648] is indicated as that used by Levy et al, 1987 [PMID:3036526] . Levy et al do not refer to any in situ probes.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Kanzler et al, 2000 [PMID:10662648] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10662648] Kanzler B, Foreman RK, Labosky PA, Mallo M 2000 BMP signaling is essential for development of skeletogenic and neurogenic cranial neural crest. Development (127):1095-104
 [ doi:10.1111/j.1432-1033.1987.tb13485.x] [ PMID:3036526] Levy E, Liem RK, D'Eustachio P, Cowan NJ 1987 Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein. Eur J Biochem (166):71-7
Links:MGI:1352811 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI