EMAGE mouse gene expression spatial database

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EMAGE:7147

Car3 carbonic anhydrase 3 ( MGI:88270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004549. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004549_01	euxassay_004549_02	euxassay_004549_03	euxassay_004549_04

euxassay_004549_05	euxassay_004549_06	euxassay_004549_07	euxassay_004549_08	euxassay_004549_09

euxassay_004549_10	euxassay_004549_11	euxassay_004549_12	euxassay_004549_13	euxassay_004549_14

euxassay_004549_15	euxassay_004549_16	euxassay_004549_17	euxassay_004549_18	euxassay_004549_19

euxassay_004549_20	euxassay_004549_21	euxassay_004549_22	euxassay_004549_23	euxassay_004549_24

euxassay_004549_25	euxassay_004549_26	euxassay_004549_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7147_wholemount_strong.wlz

7147_wholemount_moderate.wlz

7147_wholemount_weak.wlz

7147_wholemount_possible.wlz

7147_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7147_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 11 moderate expression: see section 20 21 22 23 24 25 26 27

forearm mesenchyme

strong

regional

strong expression: see section 27

shoulder mesenchyme

strong

regional

strong expression: see section 27

upper arm mesenchyme

strong

regional

strong expression: see section 01 02 26 27

foot mesenchyme

strong

regional

strong expression: see section 05

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 03 22 23 24

upper leg mesenchyme

strong

regional

strong expression: see section 01 02 03 04 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 19 20 21 22 23 24 25 26 27

esophagus

strong

regional

strong expression: see section 13 14

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24 25

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23

bladder

strong

regional

Expression in the fundus region.

kidney pelvis

strong

regional

strong expression: see section 17 18 19

male reproductive system

strong

regional

strong expression: see section 16

not examined

regional

not examined expression: see section 24

nucleus pulposus

strong

regional

strong expression: see section 14 15

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23 24 25 26 27

tail mesenchyme

moderate

regional

moderate expression: see section 11 14 15 16

axial skeleton tail region

strong

regional

strong expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7427
Entity Detected:	Car3, carbonic anhydrase 3 ( MGI:88270)
Sequence:	sense strand is shown >T7427 AGCTGTCCAGCGCTGAGAAACACGAAAGGTGACCATGGCTAAGGAGTGGGGCTACGCCAGCCACAATGGT CCTGATCACTGGCATGAACTTTATCCAATTGCCAAAGGGGACAACCAGTCACCCATTGAACTGCATACTA AAGACATCAAGCATGACCCCTCTCTGCAGCCCTGGTCAGCATCTTATGACCCTGGCTCTGCTAAGACCAT CCTGAACAATGGGAAGACCTGCAGAGTTGTGTTTGATGATACTTATGACAGGTCTATGCTGAGGGGTGGT CCTCTCTCTGGACCCTACCGACTTCGCCAATTCCATCTTCACTGGGGCTCCTCTGATGACCACGGCTCTG AGCACACCGTGGACGGAGTAAAATACGCTGCTGAGCTTCACCTGGTTCACTGGAATCCAAAGTATAACAC CTTTGGAGAGGCTCTGAAGCAGCCTGATGGCATCGCTGTGGTTGGCATTTTCCTGAAGATAGGACGGGAG AAAGGCGAGTTCCAGATTCTTCTTGATGCCCTGGACAAAATTAAGACGAAGGGCAAGGAGGCCCCTTTTA CACACTTTGACCCATCATGCCTGTTCCCTGCTTGCCGGGACTATTGGACCTACCACGGCTCCTTCACCAC GCCGCCCTGCGAGGAGTGCATTGTGTGGCTGCTGCTCAAAGAGCCCATGACTGTGAGCTCAGACCAGATG GCCAAGCTGCGCAGCCTCTTCTCCAGCGCAGAGAATGAGCCCCCGGTGCCTCTGGTGGGGAATTGGCGCC CTCCTCAGCCTGTCAAGGGCAGGGTGGTGAGGGCCTCCTTCAAGTAAGGCTCTGAGCTTGCCCTCTTCGG GCAAGAAACTCTGCCCCTGAAGAGCCTGCTTGTCTCCTCCTGGTGCTCCCTACTCCAAGCTGTCCGACAA CACCTAGAGGAAGAGGAGAAGCAGTCACATGCAACCGCAGTGCCTTTTGACATGATCTAACCCAGAAGCA TGAATTTCACACCTAACATTCTTAATAACCACCTTTTCTAAAAAAAAATAATAATAATTAGTATTTCTAG TATGAATTCAGTGAAGAAGAAATAGATGGAAAATTAGCGATAGAGAACAGTGGCTCACTGAGCATTTTAG ACTCAGAATCTCAGCTCTCCTACTTTCAGGCTGAGTTACAAATACTACAGAAGTGATGGGCCCATGCTAT GTATCCGGGTATGGAGGAGTATGCGGACCTTACCACGCTTAAGTGAAAACACACGTTAACATCATTGTAG ATCTCAAAAACTGAATTCATAATGTGTTTTACCTCAAATAATACCAGCGATAATCCATCACTCGTTAAAA ATTTGCCTACTACCAAGTTTGCCTGGTTTTAGTCACCTATAAAACACACCCATAAAGCCAGGTGTTTTAA AATGTTTGATCCAGCATTTAAATTTCTTCTTCATAAAGATGGTTTTCTTTGCCCAAAGTAGAGCCATTTA TTTTTTATTTCACTACTTTAATCTTTGCATGCCTATTAAAAACAAAAACAAACAAACAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4195712 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195712 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7150 same embryo
	EMAGE:7146 same embryo
	EMAGE:7149 same embryo
	EMAGE:7148 same embryo
	EMAGE:7145 same embryo
	EurExpress:euxassay_004549 same experiment
	MGI:4823631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source