EMAGE mouse gene expression spatial database

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EMAGE:7179

Lnp limb and neural patterns ( MGI:1918115)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002626. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002626_01	euxassay_002626_02	euxassay_002626_03	euxassay_002626_04

euxassay_002626_05	euxassay_002626_06	euxassay_002626_07	euxassay_002626_08	euxassay_002626_09

euxassay_002626_10	euxassay_002626_11	euxassay_002626_12	euxassay_002626_13	euxassay_002626_14

euxassay_002626_15	euxassay_002626_16	euxassay_002626_17	euxassay_002626_18	euxassay_002626_19

euxassay_002626_20	euxassay_002626_21	euxassay_002626_22	euxassay_002626_23	euxassay_002626_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7179_wholemount_strong.wlz

7179_wholemount_moderate.wlz

7179_wholemount_weak.wlz

7179_wholemount_possible.wlz

7179_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7179_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 02 19 20 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 03 04 06 07 22 23 24

medulla oblongata basal plate

moderate

regional

moderate expression: see section 09 17 weak expression: see section 16

facial vii ganglion

weak

regional

weak expression: see section 04 05 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 10 16

cervical ganglion

weak

regional

weak expression: see section 09 17

thoracic ganglion

weak

regional

weak expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 weak expression: see section 08 11 16 17 18 19

not examined

regional

not examined expression: see section 01 02 03 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 24 weak expression: see section 02 03 22 23

genital tubercle of male

moderate

regional

moderate expression: see section 14 15 weak expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5201
Entity Detected:	Lnp, limb and neural patterns ( MGI:1918115)
Sequence:	sense strand is shown >T5201 GGCGACCCAGGCTTCCCCGAGTGGACGCCGGGGCCGAGCGGCGCCCGCTTTTCTCAGCTGTGACTTCTTT TGGACAACTCATGATATTTATTATTGTGCCAAGTTTCTGCAAATAAAAGATGGGTGGATTATTTTCTCGA TGGAGGGCAAAACCTTCAACAGTAGAAGTTCTAGAAAATATAGATAAGGAAATTCAAGCATTGGAAGAAT TTAGAGAAAAAAATCAGAGATTGCAAAAATTATGGGTTGGAAGATTAATTATCTATTCTTCAATTCTTTA CCTGTTTACCTGTTTAATTGTGTACTTGTGGTATCTTCCTGATGAGTTTACAGCAAGACTTGTCATGACA CTCCCATTTTTTGCTTTTCCTTTGATCATCTGGACATTAAGAACTGTACTTATTTTCTTCTTTTCCAAGA GAACAGAGAGAAATAATGAAGCATTGGATGATTTAAAATCCCAGAAGAAAAAGATACTTGAAGAAGTCAT GGAAAAAGAAACTTATAAGACAGCTAAGTTAATTCTTGAAAGGTTTGATCCAGACTCAAAGAAAGCAAAG GAGTTTGAGCCGCCATCTGCTGGAGCAGCTGTAACTGCAAAGCCAGGACAAGAGATTCGTCAGCGAACTG CAGCTCAAAGAAACCTTTCCCCAGCACCAGCAAGCTCTAGTCAGGGTCCTCCTCCACAAGGTCCAGTGTC TCCTGGGCCAGCGAAGGACGCTTCAGCTCCTGGGGGACCGCCAGAAAGAACTGTTGCTCCAGCCTTGCCC AGGCGCCTTGGATCCCCTGCTACTTCAGTTCCTGGAATGGGCCTTCACCCTCCAGGTCCACCTTTAGCAA GACCCATTCTCCCCCGAGAACGAGGTGCTCTGGATAGAATTGTTGAATACTTAGTTGGTGATGGTCCACA GAACAGGTACGCCCTCATATGTCAGCAGTGTTTTTCTCACAATGGCATGGCTTTGAAGGAAGAATTTGAA TATATTGCTTTTCGATGTGCCTACTGTTTTTTCTTGAATCCTGCAAGAAAAACGAGACCTCAAGCTCCAC GGCTTCCAGAGTTTAGTTTTGAGAAGAGGCAGGCAGTGGAAGGCTCAAGCTCAACTGGTCCCACGCTACT AGAAAGTGTGCCCTCAGCAGAAAGCCAGCTCATTGAAGACTCTTTAGAAGAACAAGATGTTCTTGATAAT AGTACTGAGCAGAGAGATGACAAAATACCAGTTACAGAGCAGACAAGCCAAGTGATTGAAAAAACATCTG GTCCAGAGGAGCCAGCAGAGAACCAAGAAGAGACTGAGAACGAGGAAACCTCAACAAACGAAGCCAAGTC ACCTGTTCTCAGAGCTGATTCTGTTCCTAATCTTGAACCAAGTGAAGAATCTTTGGTGACAAAGTAGTAA ATACTTCCATGTGCCTTCAACTGGATATTTGTAGTCTTGTTGATGTTAGTTACTGCTTTTTTTTTTTTTT TGTGGTACTCAAGAAAAATTTGCCTTCTAGATATACGCTTAATATTATTCATATTCAGATTGCCCAGCAA GTTCAGTGTATCCAAGTACTACCACTAGGTATTGACAGTAAAAGCACGTGGTATCAGTTAATATGCAGAG GCATAGCTGTGCATTCTAAAGTTAAAAAAATGTTTTTTACATGTATAACTTGTAAATAATACCCACTTTG ACATAGGAACAAATTTGGCTAGGAATAATTTCTTCCATAGTATTTAAGGGTTGAAACATAATTAAGATTT TGAATACTTCATCATTAAATTTTAACTTTGGAGATATTTGCCAGTGGGAAGGAAATATCTGTTTAGGTGT TTACACCAAGTAATGCTGAAAGCTGTAACCTTAATTACATACGCATTGGTACGAATTCTCAGTAAGAATG CAACTAAACAGTCAAGTGCAGTATTTCTCTTGATGTCTTTTATTTTACAACTGGGCCAAAGGCACATAAT GTATATAGTTAATGTTTACATAGTAATAGGAAATACATCTGCACTTGACTGTACTTGAATTCTTGGTATT ATTTATATTTGTAATATAATGATGATATATAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5353629 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5353629 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7181 same embryo
	EMAGE:7183 same embryo
	EMAGE:7184 same embryo
	EMAGE:7180 same embryo
	EMAGE:7182 same embryo
	EurExpress:euxassay_002626 same experiment
	MGI:4825957 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source