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EMAGE:725

Shh sonic hedgehog ( MGI:98297)
TS12 (5 Somite no.)
in situ hybridisation

Data Images
EMAGE:725
Figure 2C of Shimamura and Rubenstein, 1997 [PMID:9226442] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Shh expression in orange, Nkx2.1 expression in purple. Only the Shh expression has been spatially mapped in this EMAGE entry. Image annotations: arrowhead - medial neural plate; hm - head mesenchyme; pr - prosencephalon; ne - neuroectoderm; ec - ectoderm; nc - notochordal plate; fg - foregut endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:725EMAGE:725Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
725_voxel_strong_3D_1.wlz
725_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:725_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
foregut diverticulum
detected detected
regionalShh is expressed in the dorsal foregut.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1335234
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Shimamura and Rubenstein, 1997 [PMID:9226442] is described as being "kindly provided from A. McMahon." Undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:5 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:alkaline phosphatase + INT/BCIP
General Information
Authors:Shimamura & Rubenstein, 1997 [PMID:9226442] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9226442] Shimamura K, Rubenstein JL 1997 Inductive interactions direct early regionalization of the mouse forebrain. Development (124):2709-18
Links:MGI:1335298 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI