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EMAGE:727

Shh sonic hedgehog ( MGI:98297)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:727
Figure 5A of Solloway and Robertson, 1999 [PMID:10079236] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:727Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
727_wholemount_strong_3D_1.wlz
727_wholemount_moderate_3D_1.wlz
727_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:727_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
Authors also report expression in the node.
future brain
detected detected
regionalExpression is in the ventral midline.
tail future spinal cord
detected detected
Authors also report expression in the node.
hindgut
detected detected
regionalExpression is in the foregut and hidgut endoderm.
foregut
detected detected
regionalExpression is in the foregut and hidgut endoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Solloway & Robertson, 1999 [PMID:10079236] is indicated as that described by Echelard et al., 1993 [PMID:7916661] as "either a 2.8 kb or 0.6 kb RNA transcript generated by T7 (2.8 kb) or T3 (0.6 kb) transcription of XbaI and HindIII digests of Bluescript (Stratagene) subclones of the original Shh cDNA inserts."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Solloway & Robertson, 1999 [PMID:10079236] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10079236] Solloway MJ, Robertson EJ 1999 Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development (126):1753-68
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:1333084 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI