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EMAGE:730

Shh sonic hedgehog ( MGI:98297)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:730
Figure 1E of Nery et al., 2001 [PMID:11171336] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: LGE - lateral ganglionic eminence; MGE - medial ganglionic eminence; POA - preoptic area.
Expression Pattern Description
Spatial Annotation:
EMAGE:730EMAGE:730Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
730_voxel_strong_3D_1.wlz
730_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:730_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression is detected in the ventral telencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Nery et al., 2001 [PMID:11171336] was previously described by Echelard et al., 1993 [PMID:7916661] as "either a 2.8 kb or 0.6 kb RNA transcript generated by T7 (2.8 kb) or T3 (0.6 kb) transcription of XbaI and HindIII digests of Bluescript (Stratagene) subclones of the original Shh cDNA inserts."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1 or Swiss Webster
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Nery et al., 2001 [PMID:11171336] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171336] Nery S, Wichterle H, Fishell G 2001 Sonic hedgehog contributes to oligodendrocyte specification in the mammalian forebrain. Development (128):527-40
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:1930433 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI