EMAGE mouse gene expression spatial database

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EMAGE:7308

Kcnc4 potassium voltage gated channel, Shaw-related subfamily, member 4 ( MGI:96670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004363. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004363_01	euxassay_004363_02	euxassay_004363_03	euxassay_004363_04

euxassay_004363_05	euxassay_004363_06	euxassay_004363_07	euxassay_004363_08	euxassay_004363_09

euxassay_004363_10	euxassay_004363_11	euxassay_004363_12	euxassay_004363_13	euxassay_004363_14

euxassay_004363_15	euxassay_004363_16	euxassay_004363_17	euxassay_004363_18	euxassay_004363_19

euxassay_004363_20	euxassay_004363_21	euxassay_004363_22	euxassay_004363_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7308_wholemount_strong.wlz

7308_wholemount_moderate.wlz

7308_wholemount_weak.wlz

7308_wholemount_possible.wlz

7308_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7308_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 15 16 17

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 21 22 23 weak expression: see section 04 05 07 08 09 16 19 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 08

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

vagus x ganglion

moderate

single cell

moderate expression: see section 08 17

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 09 15 16

cervical ganglion

weak

regional

weak expression: see section 08 09 17

dorsal root ganglion

moderate

regional

moderate expression: see section 07 14 15 16 weak expression: see section 08 09 13 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5253
Entity Detected:	Kcnc4, potassium voltage gated channel, Shaw-related subfamily, member 4 ( MGI:96670)
Sequence:	sense strand is shown >T5253 CGCTTCTTATGATCAGCTCGGTGTGTGTCTCCTCCTACCGCGGGCGCAAGTCGGGGAACAAGCCTCCGTC CAAAACATGTCTGAAGGAGGAGATGGCCAAGGGCGAGGCGTCGGAGAAGATCATCATCAACGTGGGCGGC ACGCGACATGAGACCTACCGCAGCACCCTGCGCACCCTACCGGGCACCCGCCTTGCCTGGTTGGCGGATC CCGACGGCGGGGGTCGGCCAGAGTCGGATGGCGGCGGTGCAGGCAGCAGCGGCAGCAGCGGCGGCGGCGG CGGCGGCGGGGGCTGTGAGTTCTTCTTTGATCGGCACCCGGGTGTTTTTGCCTATGTGCTCAACTACTAC CGTACGGGCAAGCTGCATTGCCCCGCAGACGTCTGTGGGCCTCTCTTTGAGGAAGAGCTCACCTTCTGGG GTATCGATGAAACAGATGTGGAACCCTGCTGCTGGATGACCTACCGGCAGCACCGCGATGCTGAGGAGGC ACTGGACATCTTCGAGAGCCCGGACGGGGGCGGGGGTGGCGCAGGGCCAGGCGACGAGGCTGGCGACGAT GAGCGGGAGTTGGCCTTGCAGCGCCTGGGCCCCCATGAAGGAGGCTCTGGCCCTGGTGCTGGGTCCGGAG GGTGCCGTGGCTGGCAGCCCCGAATGTGGGCGCTCTTCGAGGATCCCTACTCATCCCGGGCGGCCAGGGT GGTAGCCTTTGCCTCTCTCTTCTTCATCTTGGTCTCCATTACCACCTTCTGCCTGGAGACCCATGAGGCC TTCAACATTGACCGAAACGTGACGGAGATTCATCGGGTAGGGAATATCACCAGCGTGCGCTTCCGGCGGG AGGTAGAAACAGAGCCCATTCTTACCTACATCGAGGGTGTGTGCGTGATGTGGTTCACTCTAGAGTTCCT GGTTCGCATTGTGTGCTGCCCTGATACGCTGGACTTTGTCAAGAACTTGCTCAACATCATCGACTTTGTG GCCATCTTACCCTTTTACCTGGAGGTGGGATTGAGTGGTTTGTCTTCCAAGGCAGCTCGCGATGTGCTGG GTTTCCTGCGTGTGGTACGCTTTGTACGCATCCTGCGGATCTTCAAGCTCACACGCCACTTTGTGGGGCT GCGTGTGCTAGGCCACACTCTCCGGGCCAGCACTAATGAGTTCCTGTTGCTTATCATCTTCTTGGCCCTG GGTGTGCTCATCTTCGCCACCATGATCTATTATGCCGAGCGAATTGGGGCCAGGCCATCTGACCCACGGG GCAACGACCACACCGACTTCAAGAACATCCCCATCGGTTTCTGGTGGGCTGTGGTCACCATGACAACGCT TGGCTATGGGGACATGTATCCCAAGACGTGGTCAGGAATGTTGGTGGGTGCGCTGTGTGCGCTGGCTGGT GTGCTGACCATTGCCATGCCCGTGCCTGTCATCGTCAATAACTTTGGTATGTACTACTCCCTGGCTATGG CCAAGCAGAAGCTGCCCAAGAAGCGAAAGAAGCACGTACCACGGCCACCCCAGCTCGAATCGCCCATTTA CTGCAAGTCTGAGGAGACTTCACCCCGGGACAGCACCTACAGTGACACCAGCCCCCCTGCCCGGGAAGAG GGTGTGGTCGAGAGGAAACGGGCCGACTCTAAGCAGAATGGCGATGCTAATGCGGTGCTGTCTGATGAGG AGGGAGCTGGCCTCACTCAGCCCTTGGCCTTGGCCCCCACCCCTGAAGAGCGTCGGGCCCTGAGACGCTC AGGCACGCGGGACAGAAACAAGAAGGCAGCTGCCTGCTTCCTGCTCAGTGCTGGGGACTATGCCTGTGCT GATGGCAGTGTCCGGAAAGAAGGCAATGTTGAGCCGAAAGCGTGCGTCCCAGTGTCTCACACCTGTGCTC TTTAAACACAGAGACCTGCCAAGACGCCCTCTCGTCCAACTATGCCCACGCTGAAGTCCTCACCCTCTCT TAGAGTGGCGCCAACGTGAGAAAGACAGACAGACAGACAGACAGAAAGCCAGAGGCTTAGGAAAACTCTG GAACCCAGGCACGAATCTTTTGCTGGGAAAGACTCAGATATCCTTGTTTGCACAAGACTGGTGGAAAAAC CTCCCATGCGACTCTCAGGGCCCAGAGCCATCTGGGTCTGATACTCTGTTCTACTGTACATTGAAGAGAC ATATATGCACATATAGTATCTATATTCATACATACTATATACTCTTGTGTGTAGTGCACGTGCTACTGGT GGTCTGTCTTCATCGTTAGGCTATGTCTCCCAAGTCCTCTTCCCACCCTGTTTCCCCTCCTCCCTTCCTT CATGGATTGTTTCTTCTGACCACGTGTATGGGATATCCCAGGAAATGTGTCCCTGGGACCTGCCCCTCCA GCTGGGTGGTCCCAGGCTGCTCTCACTTGGGGATGTTTCCCCTGCCAGCAGGTGGCCTGTTGAAGTCAGT TGAAGGCAAGATTGCCCTCCGGGGTCACTGCTTCACTAGCCCAACCCCACCCCAGATTGGGGTTCTACCT CCCACCCCACACCCCAGTTTGTGGGGCATTTCTAGGGGCTCTTTTGCAGCCTCTGTCACTCCTTCCAATG CCCCTTCTGTGTCTGTGATAGGGGGAATTTCATTTCTTTTGTGTGTCTCCTTTGTCCATTTCTTTTCAAA GATGCTGCTGGGCAGATGGAAGGGGATCTGTTTGCCCATCTGGCTCAGGGGTCTGAGAAGGGGACACCTC GGGCGAGAGGAGACCAGTTGCAATACTGTACTTCCTGGCCAGTGGCCAGAGGATGCGTGCAATAGCAGAG GCCGGGTGACCCCTTCAGCCTTGGCCTCTGCCCCTCCCTCAGCCCTTCCTGCCCATCCTTGGTATTGGTC AGTCCTTTTCTAAAGCTGTCCTCTTGTGTGTGTGTCTGAGGCACGCCTAGGCTGGGCCCACTGTGCCCTG TTTGCATGCCCTCAACTGTCATGCTGTGCTCGAGTACCAATAAAGACATCCGAGTGTCCTGCTGCTCCTC TGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5358950 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5358950 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7309 same embryo
	EMAGE:7306 same embryo
	EMAGE:7307 same embryo
	EMAGE:7304 same embryo
	EMAGE:7305 same embryo
	EurExpress:euxassay_004363 same experiment
	MGI:4825703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source