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EMAGE:7316

Smagp small cell adhesion glycoprotein ( MGI:2448476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316
"Pseudo-wholemount" of euxassay_004382. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004382_01 euxassay_004382_02 euxassay_004382_03 euxassay_004382_04
EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316
euxassay_004382_05 euxassay_004382_06 euxassay_004382_07 euxassay_004382_08 euxassay_004382_09
EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316
euxassay_004382_10 euxassay_004382_11 euxassay_004382_12 euxassay_004382_13 euxassay_004382_14
EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316
euxassay_004382_15 euxassay_004382_16 euxassay_004382_17 euxassay_004382_18 euxassay_004382_19
EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316 EMAGE:7316
euxassay_004382_20 euxassay_004382_21 euxassay_004382_22 euxassay_004382_23 euxassay_004382_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7316Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7316_wholemount_strong.wlz
7316_wholemount_moderate.wlz
7316_wholemount_weak.wlz
7316_wholemount_possible.wlz
7316_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7316_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 17 18 19 21 22 23 24 weak expression: see section 01 02 03 04 05 06
humerus
moderate moderate
regionalmoderate expression: see section 04 05 21 22 23 24 weak expression: see section 01 02 03
hindlimb digit 1 mesenchyme
moderate moderate
regionalmoderate expression: see section 22
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 23
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 23
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 22
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 22
lower leg mesenchyme
moderate moderate
regionalmoderate expression: see section 23 24 weak expression: see section 01 02
upper leg mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05 17 18 19 20 21 22 23 weak expression: see section 01 02 03
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 13 14 15 16 17
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 07 09 10 11 13 14
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 09 10 11 13
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 07 09 10 11 13 14 15 16
scapula
weak weak
regionalweak expression: see section 03 04 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6401
Entity Detected:Smagp, small cell adhesion glycoprotein ( MGI:2448476)
Sequence:sense strand is shown

>T6401
CGCTGCGCGGAAGCCCGGAGCCCGCACTCGGCCGCCGCGCGCCTGCGACCTGACCCGCACGGGTACTGCG
CGGCTGCAGGGACACTCGACGGCCAGGGCGCGGGCCCGGGGCACGCGGGATCCCCGGGGAGCGGGGCGCA
CCCAGCGGACGGCTCGGCGGGGCTCCTGGCCCTGGAAGCCCAGATCTGACCCTACGAAATAGCCTGCCCT
CAGCAACGATGAACAACCTACCGGCCACACCTTCTCCTGAAGAATTGATGACCACGCCGGTTTTTCAGGC
CCCTGAGACCCTGTCCCCACAAGCTGAAGAGGCCAGCACAGCGCTCATTGCAGTTGTTATCACCGTGGTG
TTCCTCACCCTGCTCTCGGTGGTGACCTTGATCTTCTTTCACTTGTACAAGAACAAAGGCAGCTACGTCA
CCTATGAGCCTGCAGAAGGGGAGCCCAGCGCCATCCTCCAGATGGAGACTGACTCAGCCAAGGGCAGAGA
GAAGGAAGAGTACTTCATCTAATGCTTCCCAGGCTGGAGGGGCCAATTCTTGGCTCCAACACTAAGCCGC
TGCCTCTGTAGTTAGGGAACGTTTGCTCTAAAGCCAGGGAGTGGCGTTGGGTGATACTGGCACATCCACT
CACCTCCCAGGACACAGCCCCCAATACCGGAATCACTGACTCCAGGGTCCAGAGACACGGAGAAAGCTGT
TCATGATGCTGGGCCTTGATAAGGACAGTGCTCGAAACCGACCACCAAAGAGGGGCCATGCCTGAGTTGG
AAGTGAGGTCACATGCTGGTCCACTTTGTCCCCTCCCTAGTTGACGACCAATAGCCCCAGTCAGTGCTAT
CCAGTCTTTCTGGAGGCAGGACACCACAGGGAGGGGTCAGACCAGGGAGGGGATAGGAGTCTGAGAATGT
TACTGCCTTTGTTTGATCTGAGCCCCACTGTTCCCCAATCAGCCTCAAACTTCTACTGTTTTTATCCTAA
TTAAACTTATGAAAATCAGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3582955 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582955 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7317 same embryo
 EMAGE:7318 same embryo
 EMAGE:7315 same embryo
 EMAGE:7314 same embryo
 EMAGE:7319 same embryo
 EurExpress:euxassay_004382 same experiment
 MGI:4828317 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS