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EMAGE:7383

Ppa1 pyrophosphatase (inorganic) 1 ( MGI:97831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383
"Pseudo-wholemount" of euxassay_004470. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004470_01 euxassay_004470_02 euxassay_004470_03 euxassay_004470_04
EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383
euxassay_004470_05 euxassay_004470_06 euxassay_004470_07 euxassay_004470_08 euxassay_004470_09
EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383
euxassay_004470_10 euxassay_004470_11 euxassay_004470_12 euxassay_004470_13 euxassay_004470_14
EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383
euxassay_004470_15 euxassay_004470_16 euxassay_004470_17 euxassay_004470_18 euxassay_004470_19
EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383 EMAGE:7383
euxassay_004470_20 euxassay_004470_21 euxassay_004470_22 euxassay_004470_23 euxassay_004470_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7383Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7383_wholemount_strong.wlz
7383_wholemount_moderate.wlz
7383_wholemount_weak.wlz
7383_wholemount_possible.wlz
7383_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7383_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
forelimb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 23 24 not examined expression: see section 09
hindlimb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23 24
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19 20
pancreas
moderate moderate
regionalmoderate expression: see section 06 07 08 09
vibrissa
moderate moderate
regionalmoderate expression: see section 05 06 07 08 19 20 21
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 16
nasal septum
moderate moderate
regionalmoderate expression: see section 14 15
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
midgut
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20 21
mandible
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16
maxilla
moderate moderate
regionalmoderate expression: see section 06 07 08 17 18 19 20
palatal shelf
moderate moderate
regionalmoderate expression: see section 09
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16
bladder
moderate moderate
regionalmoderate expression: see section 11 12 13 14
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 09 16 17 18 19
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
cranium
moderate moderate
regionalmoderate expression: see section 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23
tail
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16
not examined not examined
regionalnot examined expression: see section 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6456
Entity Detected:Ppa1, pyrophosphatase (inorganic) 1 ( MGI:97831)
Sequence:sense strand is shown

>T6456
GTGTGGCTGTGCAGCGGCGGCGGCGGTGCATCTCCGACACCATGAGCGGCTTCAGCAGCGAGGAGCGCGC
CGCGCCCTTCACCCTCGAGTACCGCGTCTTCCTCAAAAATGAGAAAGGGCAGTACATTTCTCCATTTCAT
GATGTTCCAATTTATGCAGACAAGGATGTGTTCCACATGGTGGTTGAGGTTCCACGCTGGTCCAACGCCA
AAATGGAGATTGCTACAAAGGACCCTTTAAACCCAATCAAGCAAGATGTGAAAAAGGGGAAGCTCCGCTA
TGTGGCGAATCTGTTCCCTTATAAAGGGTATATTTGGAACTACGGCGCCATCCCTCAGACATGGGAAGAC
CCAGGACACAGTGACAAGCACACTGGCTGCTGTGGTGACAACGACCCAATCGATGTTTGTGAAATCGGAA
GCAAGGTGTGTGCCAGAGGTGAGATAATCAGGGTGAAGGTCCTGGGCATACTGGCCATGATCGATGAGGG
CGAGACCGACTGGAAGGTCATTGCCATTAATGTGGACGATCCGGACGCAGCCAATTATAAAGATATTTCT
GATGTCGAACGGCTAAAACCCGGCTATTTAGAAGCTACTGTTGATTGGTTTAGAAGGTATAAGGTTCCTG
ATGGAAAACCAGAAAATGAGTTTGCATTCAATGCAGAATTTAAAAACAAGGACTTTGCAGTTGACATTAT
TAAAAGCACCCACGATTATTGGAAAGCATTAGTGACTAAGAAAACAGATGGGAAAGGAATCAGTTGCATG
AACACCACGGTGTCGGAGAGCCCCTTCAAGTGCGATCCTGATGCTGCCAAAGCCATTGTGGATGCTTTGC
CACCACCATGTGAATCTGCCTGCTCACTGCCGACAGATGTGGATAAATGGTTCCATCAGCAGAAAAACTG
AAGAGCTTCCTCTGGAACACGAGCTGTTACGGCCGCATCCTGTTCACCTGGAAGCATTAGATGCAAAAGA
TTTTCCAAGCTCTAAATTTCTGTAGCTCACCGAATGAAGTAGACTCTGCTGTGACTGGTCCACTAGTTCA
GAACTGGTTCAGAAGGTTAGCCATCTAGACACCTCACATCTCAACCAAGACAGCTTTTATTAAACCTTAG
CTCTCAAAGCCGTGGGGACATGGAAGTCATTTGTGCATGGATGTGCAGTGTGGACACCTATTGGATGTAC
ATGTTTACTGTGTTAGGAAATAAAATTATTTTACTGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3585780 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585780 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7387 same embryo
 EMAGE:7385 same embryo
 EMAGE:7382 same embryo
 EMAGE:7386 same embryo
 EMAGE:7384 same embryo
 EurExpress:euxassay_004470 same experiment
 MGI:4827345 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS