EMAGE mouse gene expression spatial database

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EMAGE:7387

Asns asparagine synthetase ( MGI:1350929)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004453. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004453_01	euxassay_004453_02	euxassay_004453_03	euxassay_004453_04

euxassay_004453_05	euxassay_004453_06	euxassay_004453_07	euxassay_004453_08	euxassay_004453_09

euxassay_004453_10	euxassay_004453_11	euxassay_004453_12	euxassay_004453_13	euxassay_004453_14

euxassay_004453_15	euxassay_004453_16	euxassay_004453_17	euxassay_004453_18	euxassay_004453_19

euxassay_004453_20	euxassay_004453_21	euxassay_004453_22	euxassay_004453_23	euxassay_004453_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7387_wholemount_strong.wlz

7387_wholemount_moderate.wlz

7387_wholemount_weak.wlz

7387_wholemount_possible.wlz

7387_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7387_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

pancreas

strong

regional

strong expression: see section 06 07 08 09 10 11

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex mantle layer

strong

regional

strong expression: see section 09 10 11 14 15 16 17 moderate expression: see section 08

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 14 15 16 17 moderate expression: see section 08

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 11 13 17 18 moderate expression: see section 05 06 07 08 12 14 15 16 19 20

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 weak expression: see section 16

pons mantle layer

strong

regional

strong expression: see section 08 18 moderate expression: see section 05 06 07 09 10 11 12 13 14 15 17 19 20 21 22 weak expression: see section 16

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 09 19 21 22 moderate expression: see section 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 moderate expression: see section 20

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 17 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 09 19

trigeminal v nerve

strong

regional

strong expression: see section 09 moderate expression: see section 17

spinal cord floor plate

strong

regional

strong expression: see section 10 11 12 14 15 16

spinal cord mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 12 14 15 16 17 18 19 20

retina

strong

regional

strong expression: see section 01 02 03 22 23 24

naso-lacrimal duct

strong

regional

strong expression: see section 22 weak expression: see section 21

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 15 17

lower jaw incisor

strong

regional

strong expression: see section 11 12 15 16

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail dorsal root ganglion

moderate

regional

moderate expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6455
Entity Detected:	Asns, asparagine synthetase ( MGI:1350929)
Sequence:	sense strand is shown >T6455 GCGGCCTCCAACCGGTCTTGTCACTGCGCTGCCTCTGCTCCACCTTCTCTGGCCCTGGCCGCTAGTGCTC AGAGTGCCTGCAGTCCGCCTGTAGCATGTGTGGCATCTGGGCCCTCTTCGGCAGCGATGACTGCCTTTCC GTGCAGTGTCTGAGTGCGATGAAGATCGCGCACAGGGGGCCAGATGCATTTCGCTTTGAGAATGTCAATG GATACACCAACTGCTGCTTTGGCTTTCACCGCTTGGCTGTGGTTGACCCGCTGTTTGGAATGCAGCCGAT AAGAGTGAGGAAATACCCTTATTTGTGGCTCTGTTACAATGGTGAAATCTACAACCACAAGGCGCTACAG CAACGTTTTGAATTTGAATATCAGACCAATGTGGATGGTGAGATTATCCTCCACCTCTATGACAAAGGAG GCATCGAGAAAACCATCTGTATGCTGGACGGGGTGTTTGCATTCATCTTACTGGACACTGCCAATAAGAA AGTATTTCTGGGCAGAGACACCTATGGAGTCAGGCCCTTGTTTAAAGCCATGACAGAAGATGGGTTTCTG GCTGTGTGTTCAGAAGCTAAAGGCCTTGTTTCCTTAAAACACTCCACCACTCCCTTCTTAAAAGTGGAGC CCTTCCTTCCTGGACACTATGAAGTTTTGGATTTAAAACCAAATGGCAAAGTTGCGTCTGTGGAAATGGT CAAATACCATCACTGTACGGATGAACCATTGCATGCCATCTATGACAGCGTGGAGAAACTCTTCCCAGGC TTTGACCTAGAGACCGTGAAGAACAATCTGCGTATCCTTTTTGACAACGCTATCAAGAAACGCTTGATGA CAGACCGGAGGATTGGCTGCCTTTTATCAGGGGGCCTGGACTCGAGCTTGGTTGCTGCCTCTCTGCTGAA GCAACTCAAGGAGGCCCAAGTTCAGTATCCTCTCCAGACATTTGCTATTGGCATGGAGGACAGCCCCGAT CTCCTGGCCGCTAGAAAGGTGGCAAATTATATTGGAAGCGAGCATCATGAAGTCCTTTTTAACTCTGAAG AAGGCATTCAGGCCCTGGATGAAGTCATATTTTCCTTGGAAACTTATGATATTACGACAGTTCGGGCATC TGTGGGCATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGACAGCGTGGTGATCTTCTCCGGAGAG GGGTCAGATGAACTTACACAGGGCTATATATATTTCCACAAGGCTCCTTCCCCTGAGAAGGCCGAGGAGG AGAGTGAGAGACTGCTGAAGGAACTCTACCTGTTTGATGTTCTCCGGGCCGACCGCACTACTGCTGCACA CGGTCTCGAACTGAGAGTCCCCTTTCTGGATCATCGGTTTTCTTCCTATTACCTGTCTCTGCCGCCAGAT ATGAGAATTCCAAAAAATGGCATAGAAAAACATCTCCTGAGAGAGACTTTTGAGGACTGCAACCTGCTAC CCAAAGAGATTCTCTGGCGACCCAAAGAAGCCTTCAGTGATGGGATCACCTCAGTCAAGAACTCCTGGTT CAAGATTTTGCAGGACTATGTTGAACATCAGGTTGATGATGAGATGATGTCTGCAGCCTCCCAGAAGTTT CCCTTCAATACTCCCAAAACTAAGGAAGGCTACTTCTACCGTCAGATCTTTGAACGCCATTACCCAGGCC GGGCTGATTGGCTGACTCATTATTGGATGCCTAAGTGGATCAATGCTACTGACCCTTCTGCCCGCACTCT GACCCATTATAAGTCAGCTGCCAAAGCTTAGGCACTCTCTACACTCTTGTGTAAAAGTAAATGTTTCTTC CGGCTCTGAAGGTCGAGACAGCGACACAATCAGAAAGAATGAGACTCAGCCATCAGTCACCCAGGCTTAC TTAGGCATGAAAAGAAATAAAAGTTTCACATCTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585775 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585775 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7385 same embryo
	EMAGE:7382 same embryo
	EMAGE:7386 same embryo
	EMAGE:7383 same embryo
	EMAGE:7384 same embryo
	EurExpress:euxassay_004453 same experiment
	MGI:4823278 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source