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EMAGE:740

Shh sonic hedgehog ( MGI:98297)
TS15 (24 Somite no.)
in situ hybridisation

Data Images
EMAGE:740
Figure 6C of Ishibashi & McMahon, 2002 [PMID:12361972] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrow indicates first evidence of a dorsal expansion, at the presumptive ZLI; arrowhead indicates diencephalon-midbrain boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:740Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
740_wholemount_strong_3D_1.wlz
740_wholemount_moderate_3D_1.wlz
740_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:740_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalFirst evidence of dorsal (lateral) expansion, at the presumptive ZLI.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2676492
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Ishibashi & McMahon, 2002 [PMID:12361972] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:24 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Ishibashi & McMahon, 2002 [PMID:12361972] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:12361972] Ishibashi M, McMahon AP 2002 A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development (129):4807-19
Links:MGI:2676617 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI