EMAGE mouse gene expression spatial database

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EMAGE:7412

Fbxw24 F-box and WD-40 domain protein 24 ( MGI:3646659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005222. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005222_01	euxassay_005222_02	euxassay_005222_03	euxassay_005222_04

euxassay_005222_05	euxassay_005222_06	euxassay_005222_07	euxassay_005222_08	euxassay_005222_09

euxassay_005222_10	euxassay_005222_11	euxassay_005222_12	euxassay_005222_13	euxassay_005222_14

euxassay_005222_15	euxassay_005222_16	euxassay_005222_17	euxassay_005222_18	euxassay_005222_19

euxassay_005222_20	euxassay_005222_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7412_wholemount_strong.wlz

7412_wholemount_moderate.wlz

7412_wholemount_weak.wlz

7412_wholemount_possible.wlz

7412_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7412_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

strong

spotted

strong expression: see section 07 08 20 21 moderate expression: see section 16 17 18 19

telencephalon mantle layer

moderate

spotted

moderate expression: see section 16

telencephalon meninges

strong

spotted

strong expression: see section 01 05 06 07 08 20 21 moderate expression: see section 02 03 04 15 17 18 19

hindbrain meninges

strong

spotted

strong expression: see section 05 06 07 08 12 13 14 moderate expression: see section 03 04 15 16 17 18 19

midbrain meninges

strong

spotted

strong expression: see section 05 06 07 08 moderate expression: see section 03 04 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7628
Entity Detected:	Fbxw24, F-box and WD-40 domain protein 24 ( MGI:3646659)
Sequence:	sense strand is shown >T7628 AAGAGGTAATCCGTGGACTTAGGCCACTGAAGATGGAGATCCATTTGTCTAGTTTCCCTATGATGGAAAT CTTCTCCTATCTAGATGCCTACAGTTTGCTACAGGTTGCCCAAGTGAACAAGAACTGGAATGCACTTGCA AGCAATGATTTCCTGTGGAGGAAGTTGTGTCAGGAAAGATGGCTCTTCTGTGACATGGTTACCCTACAGC TCCTGGGCAAAGAGACATGGAAACAGTTCTTTGTTTACCGAACATGGCAAGAACACGTCAAGTCCCGGGC AATTCCAGAAGATTTCACTTACAAGGAGATTCCTTTGGAGTGTGGTGTTCGGGGATATGCAGGTTACATA TCAGGCTGTGCCTTAACAAGAAACGGACAAGGCAAGTCTGTTGTCTGTATGGTGAGTTCCAAGAACAAGA TTTCCACCTGGGATATTAGCGAGAGCGTTATAACCTGGGTAAGCCCAGTACAGCCAGCAAGCATCAAGTT GTTGACTACCCTCCCTGATATGCACATTGCGGTCACTGTAGATATACAATCAACTATCAAACTGTGGGAC TGTCACAACAGGGAAGCTCTGGCAACTAACAACCTGGAATCTCCATGTAAATCACTGAAAGCTGTCATTT CCAAGGATGGCCCAATTGTCTTGGCAGGCGATATCTTGGGTAACCTCTACATCTTTAGGATTCCAGATTT ACACCTCATTTCCACAGTCAATGTATTCCCTTGCGGTTTTGATAAAATTGATTGCTCTCCTCAGAAGAAA TGGGTCTTACTGAGTCAGAACCACCCATGTATTTATCCAAAGGTGTTTTACATGAGCAGCTTACTGAGGA CATCAGAATTCTCTGACCCAGTGTCTACCGTTCTCGAATTTTCCTTATGCAAAAGAGCCTTCTGGACCCC AAGAAGGGAAGACAGAACAACACTGATGTCCAGAAGAGGGCGCCCCATAGTCACAAGGTTTGAAACATTT GATATGAAGCTGGAAGAGAACGGGAACAAAACAATTGTTAAAGGAGATTTGGTAGCGAGTTTCTCTTTGC AAGACTACAATGAGAACCCGAAATGGATGGGAGTCAGTGATAAGAGCGTGATTGTTTGTTCAACTGGATC CTCTCTCTTGCTCTTCAGCATGAAGGGTCTCCATCTGCAGACATTTCAGTACAGCCCAGAAATGATTGTG AGACTATGGGTGGACCCCGTTCATGTCATCATCACCTGTAATGATGGCTCTATAGATGTGTACATGTGGG AGGAGAGAAGCCTATTGCTCAAGATGTGTTATCGACTGCAAAACGGGAGACATCTGCCACCATTTGGCTT CATTAAAAATTTGCTATGTGATGATGTGAGCATAGTTCAGTTGATGATAGATCGTCAAGGCCCCTGCTTT CTGATGGCATATACCTTGAACATCTGCTCTTGAAAACTGAGTGGGTTGTGTGTATTCTTCCTCAATGATA ATAAAGGTCTATTTGCAAGCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30473126 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30473126 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7413 same embryo
	EMAGE:7414 same embryo
	EMAGE:7416 same embryo
	EMAGE:7417 same embryo
	EMAGE:7415 same embryo
	EurExpress:euxassay_005222 same experiment
	MGI:4824815 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source