EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7602

Smpd3 sphingomyelin phosphodiesterase 3, neutral ( MGI:1927578)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004706. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004706_01	euxassay_004706_02	euxassay_004706_03	euxassay_004706_04

euxassay_004706_05	euxassay_004706_06	euxassay_004706_07	euxassay_004706_08	euxassay_004706_09

euxassay_004706_10	euxassay_004706_11	euxassay_004706_12	euxassay_004706_13	euxassay_004706_14

euxassay_004706_15	euxassay_004706_16	euxassay_004706_17	euxassay_004706_18	euxassay_004706_19

euxassay_004706_20	euxassay_004706_21	euxassay_004706_22	euxassay_004706_23	euxassay_004706_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7602_wholemount_strong.wlz

7602_wholemount_moderate.wlz

7602_wholemount_weak.wlz

7602_wholemount_possible.wlz

7602_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7602_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 01 02 03 23 24

fibula

strong

regional

strong expression: see section 01 23 24

tibia

strong

regional

strong expression: see section 01 23 24

femur

strong

regional

strong expression: see section 01 02 03 04 19 20 21 22 23

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 08 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 18 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 17

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 13 14 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 14 15 16 17

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

mandible

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

lower jaw incisor

strong

regional

strong expression: see section 11 12 15 16

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

palatal shelf

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

axial skeleton

strong

regional

strong expression: see section 08 09 10 11 15 16 17 18 not examined expression: see section 14

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

not examined

regional

not examined expression: see section 02 03

clavicle

strong

regional

strong expression: see section 09 10 17 18 19 20 21 22

scapula

strong

regional

strong expression: see section 02 03 04 22 23 24

pelvic girdle skeleton

strong

regional

strong expression: see section 07 08 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8647
Entity Detected:	Smpd3, sphingomyelin phosphodiesterase 3, neutral ( MGI:1927578)
Sequence:	sense strand is shown >T8647 CGCGGAGCTGTTCGCCGCTCCAGGTGCTGAGTCCGAGGGAGGCTCCGGATGCCAGAGCCGCGGTCGCTGT AACCATCGCCGCCGCGAGAGCCGTCTCTAGGAAGCCCCGGTCGCGAGGAGCTGGGAGGCGCCGCGTGCGC TGCCCCGCACCGAGCGCCCTCCTGTGCGCCACCTGAGAAAAGAAAAGAAGAGAAGAGAAGAGAAGAGAAG AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAAAAAACACAACAGTTAAAAGGTTTTCAGCAGACTCC GGTGTCCCAGCATGGCCCCTTGAAGCGAACCATGACTGTCTGTGGGACACTCGCCACTGATCATCCAGAG TCACATCTCACCAGGAGGTGACCTCTCCTCCATCCTCCACCTGCCCCCTGCCTGGGACAGTGCTGCGGCT GCCACGGACACCATGTAGTAGAGCCGGCTGCGGCGCCCAGTGAGCTGCAATGGTTTTGTACACGACCCCC TTTCCTAATAGCTGTCTGTCTGCCCTCCACGCCGTGTCCTGGGCCCTCATCTTCCCATGTTACTGGCTGG TGGACCGGCTGTTGGCCTCTTTCATACCCACCACCTATGAGAAGCGCCAGAGGGCAGATGACCCCTGCTG CCTGCAGCTGTTCTGCACGGTCCTCTTCACGCCAGTCTACCTGGCTCTGCTTGTGGCTGCACTGCCCTTT GCCTTTCTCGGGTTCATCTTCTGGTCTCCACTGCAGTCTGCCCGCCGGCCCTACTCCTACTCCCGGCTAG AAGACAAGAACCCAGCTGGTGGGGCAGCCCTGCTTAGTGAATGGAAGGGCACGGGGGCAGGCAAAAGCTT TTGCTTCGCCACGGCCAATGTCTGCCTCCTCCCCGACTCACTCGCGAGGCTCAATAATGTTTTCAACACT CAAGCTCGGGCCAAAGAGATTGGGCAGAGAATCCGCAATGGGGCCGCCAGGCCTCAGATCAAGATCTACA TCGATTCTCCCACCAACACCTCCATCAGTGCAGCCAGCTTCAGCAGCCTGGTGTCGCCACAGGGTGGTGA TGGATCCAGAGCTGTTCCTGGGAGCATTAAGAGGACAGCCTCTGTGGAATACAAGGGGGATGGTGGGCGT CACCCCAGCGACGAGGCTGCCAACGGCCCAGCCTCTGGAGAACAGGCTGACGGAAGCCTTGAGGACAGCT GCATTGTGCGAATTGGTGGCGAGGAAGGAGGTCGGCCACAAGAAGCTGATGACCCTGCTGCCGGGAGCCA AGCCAGGAATGGGGCTGGTGGGACCCCAAAGGGCCAGACACCCAACCACAATCAGCGAGATGGGGATTCT GGGAGCCTGGGCAGCCCCTCAGCCTCCAGGGAGTCCTTGGTGAAGGCACGGGCTGGGCAAGACAGTGGTG GCAGCGGAGAGCCAGGGGCTAACAGCAAGCTGCTGTATAAGACCTCTGTGGTGAAGAAGGCAGCAGCCCG CAGGAGGCGCCACCCTGATGAAGCCTTTGACCACGAGGTCTCAGCCTTCTTCCCGGCCAATCTGGACTTC CTGTGCCTGCAGGAGGTGTTTGACAAGCGTGCGGCTGCTAAGTTGAAAGAGCAGCTACACGGCTACTTCG AGTACATCCTGTATGATGTTGGGGTCTACGGTTGTCATGGTTGCTGCAATTTCAAATGTCTCAACAGCGG TCTCTTCTTCGCCAGCCGCTACCCTGTCATGGACGTGGCCTATCACTGTTACCCCAATGGGTGCAGCTTC GATGCCCTGGCCTCTAAGGGAGCTCTGTTTCTCAAGGTGCAGGTGGGAAGCACACCTCAGGACCAAAGAA TTGTTGGGTACATCGCCTGCACACACCTGCATGCCCCACCAGAGGACAGTGCCGTCCGGTGTGAGCAGCT GGACCTGCTTCAGGACTGGCTGGCTGATTTCCGAAAATCTACCTCCTCGACCAGCACAGCCAACCCCGAG GAGCTGGTGGTGTTTGACGTCATCTGTGGAGATTTGAACTTTGATAACTGCTCTTCTGATGACAAACTGG AGCAGCAGCACTCGCTCTTTACTCGCTACAAAGACCCCTGCCGCCTTGGGCCCGGGGAGGAGAAGCCTTG GGCCATTGGCACCCTGCTGGACACAAACGGTCTCTATGATGAGGATGTGTGTACCCCGGACAACCTTCAA AAGGTGTTGGAGAGCGAGGAAGGCCGCAGGGAGTATCTGGCCTTCCCCACCAGCAAGAGCCCAGGTGCTG GGCAGAAGGGCCGGAAGGATCTGCTGAAGGGCAATGGCCGCCGCATCGACTACATGCTGCACGCCGAGGA GGGGCTGTGCCCTGACTGGAAGGCTGAGGTAGAAGAATTCAGTTTTATCACCCAGCTGTCCGGCCTGACC GACCACCTCCCCGTGGCCATGCGGCTGATGGTGTCTGCAGGGGAAGAGGAGGCATAGACCAGCCATGCCA CGGTCTCGAGCAGTAGGGCCTCTGCCGGCCCTTCAAGCCACAGCCCGTGCCTGGGCTGTGTGCCCTCCAT TGAGCACCCAGTGCTGGGGGAAGGAGGGCAGGGGACAGGGAAGGACGAGCCCATCAATCCCCTGCAAGCC TGGGAACCAGGCTGCTCTGTGCCTCTGGGCCAACTCTGCGGACAGAGGACTTGGGCCAGCCTGGGAGCCC CCGGCTGCCTGACCAGTGCCATTCTTTCAGTCACGATTTTCTACAGAAATACACAGCACAACCTAGTTTG TAACCCAAGGGTTAGTACGAGGACCGGGTTTCTGTACTCTTTGTATCTCTTCTCAAGCTTTGTCCAGGGT TCATTACTTGTGTCTGCTGCCAATGTTGTCTCGCATGCCTGCACCCTCGCATGCCCACTGCCCGCATGCC ATGTGCCACACTGTAGCACCCACACCCCTTGCTCGGGCCTCAAAGGCCAAACTCAAAACACAAAAAACAC AATCAGAACCAGCCAAAGAAGCACTTCCTGGGCACAGCTGCCAGCTCTCCCCCCTCCAGCCTGGTGGGGA GTGATTGACGGCTGTCATTACCTGCCAGCCCAGGGCTCTTCCCAGGGTCTTGCATTCAAGGGCGATTACA TTTTAAGAAGAAAACAGAAAAAAATGTTACACACAAAATCACCCTTCCCTTAGGGTCTGTAAGTCACTGG GTAGGGCTGTGCTCTTTCCCAGCCCCCACAGCAGGGTCCAGCTGGCTCTGGCCACCCCAGCAGCTCCTAT AGGAGACTCATACCAGCTCTGGGGCCCTAACAGGAAATCCAAGAGGGCATCACTTTTGTACACGAAGGGG ACCCAGAATATAAGGGGGTGGGGGTTCTGATTTTGAAACTGACAGGTTCGTAGCTCCTTCTCTCCTACCC AACATGTCCTCTTCTGTGGGGCCCTTTGGGTTAGCCTCTTCCTGAGGACTTCCCTCATCCATGTATGGCT GAGCTCAGGAACCACTTGCCTGCCCCCAAGTCAGGCTGCGCCTCTGGCCTCCCAGGCCCACCCTTCCACA ATGGCTACCCTGGATAAAGTGGCAGGAAACCAAGATAGAATGGCTTCTTTACCACTGCAAGCTCAGGTGG CCAGCCAACAGCCACTGCTTGTCTAGAGCAGGGCTGACTCCAGACAGCATCCAGAAGCCACTCTTGCCAA CGTGTGACTAGAGCCAGTCCCAAGCTCAGCACAGTAAGGCTGCTAGAAGCCCACTGCTCTCCACCTGGTC CTGCTCAGGTGCCCAAGAGCACTGATCCTGCCGACCTCCCTTCAGAGAACGGAGGCTGATCCCTGACCAC ACAGGAAGGGACGACGTCTGCCTCAGGCTGCTCAGCTACTGATTTGAGGGACTATTTGGGTATTCCCAAC GGTCTCTTCAGAGTGCTCTGGTCTTGTATCTGGCCTGGCGATGATGGAGGGAGCATCAGGCCCCTGTGGT TGGGACCTCTAAAATGTTGGTGCCCTGAAGCTGTGCCTTGTCACCCACCAAGTAGCAAGAGGGATCCAGA GGGGCACACATTTGTCAGACGCCAGGCTGTGCTGAGCAGTGACCTTCAGAGTATGGGCAGTTTAGTATTA GGGCATGGATAGCTGGAGACACTGGTCTCTTAACCTCTTGGCCTAGAGAACCCTGCCCATGCTCCAGTGT CCAGAGCTAGGACAGCCTTGCTGGGTTCTCTGCTGTGCCCCTCTTCTGCCAGGGCCTGGCCTCCCAGGAA TCTGGGAAGCCCTCATGGAGTAAAAGGCCCCTAACTCCTGGAAACAACCAGGATCCTATCCATTCAAGGT ACCATGGGGCAGGTCCAATCTTCAAACCAATGGCACTGTGCACAAGGAGAGTAGAGGAGGCCGAGGCCAC CGTCAGGGTCCCTAAACCTGGGTTAGGGTAAAATGTTTGGAGTAAGAGCCGCATCCTTCTCTCCTTCCCA ACTCCCAGGGCAGCCTTAGGCAAGGTGTACTGCCCTGTGCCTCTGTTATCACAAGGTCACCTCTACACGG TCCGGTTCTTGAATCCAGAGGAGGGACAGCCTGCCTGCCTTCAGGTCCCCTACACATCTCAGCACTGAGG CCATGCCCGTCTGCCACACACTCCGCTGTGGTTTGCCTGTAGACTCAGGGTTCCTGTACTGCCAGGCCTT TCTATTTCAACTTTTAAAACAGAAGAGGCGTTGCTAGGATCCCAGACTCTCTGACATCTAAGGCCGACAC AGGGATCACCTGACTCCTAACCGGCTCTGCCTGCCCAGGACCTCTCAAGGGAAGCATAGCGAAGTGTACT AGCTTAGCAAGGACCATTCAATTCATCTTTATTTGTCCAACACCACCTGGAGTTTTAAGTATGAATATTA ACCTTGATGCTTTTTAACTATTGTATTAACTTGCTCAGATTTAGAAATACTGTTTTAAAAGACAAAGAAG AAACTTTTTTAATTTCTGTATTTTTTTTCTGTATTGTATCCTATGGGACACTAGGGGTTTTATATGGTAA GACACCCAAGGTTTTGGTAAAACATTATCAAGTATATATCCAGACAATTCTTCCCTAGAAGAAAAAACAA TCTCTATGCCTGATTTAAAAAAAAAGTCGAAAAGAGGTGGATTTTCCCTTTATGGTGCTGAAAGGAAGAC GGAGACTGAGGAGAAAATAAACCCGTGAGGAGGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5721118 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5721118 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7601 same embryo
	EMAGE:7604 same embryo
	EMAGE:7606 same embryo
	EMAGE:7603 same embryo
	EMAGE:7605 same embryo
	EurExpress:euxassay_004706 same experiment
	MGI:4828332 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source