EMAGE mouse gene expression spatial database

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EMAGE:7641

Trim62 tripartite motif-containing 62 ( MGI:1914775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004737. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004737_01	euxassay_004737_02	euxassay_004737_03	euxassay_004737_04

euxassay_004737_05	euxassay_004737_06	euxassay_004737_07	euxassay_004737_08	euxassay_004737_09

euxassay_004737_10	euxassay_004737_11	euxassay_004737_12	euxassay_004737_13	euxassay_004737_14

euxassay_004737_15	euxassay_004737_16	euxassay_004737_17	euxassay_004737_18	euxassay_004737_19

euxassay_004737_20	euxassay_004737_21	euxassay_004737_22	euxassay_004737_23	euxassay_004737_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7641_wholemount_strong.wlz

7641_wholemount_moderate.wlz

7641_wholemount_weak.wlz

7641_wholemount_possible.wlz

7641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 15 16 17 18

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 05 weak expression: see section 04 06 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 16 17

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 16 17 18

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 14 15 16 17 moderate expression: see section 09 10

retina

moderate

regional

moderate expression: see section 01

neural retina

moderate

regional

moderate expression: see section 02 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 14 15

maxilla

strong

regional

strong expression: see section 11 13

palatal shelf

moderate

regional

moderate expression: see section 10 11 12

sternum

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8910
Entity Detected:	Trim62, tripartite motif-containing 62 ( MGI:1914775)
Sequence:	sense strand is shown >T8910 CGGGCTCCGCTTCGCGCCGCCCCTGGCGCCATGGCGTGCAGCCTCAAGGACGAGCTGCTGTGCTCCATCT GCCTGAGCATCTACCAGGACCCGGTGAGCCTGGGCTGCGAGCACTACTTCTGCCGCCGCTGCATCACCGA GCACTGGGTGCGGCAGGAGGCTCAGGGCGCCCGCGACTGCCCGGAGTGCCGGCGCACGTTCGCCGAGCCC GCGCTCGCGCCCAGCCTCAAGCTGGCCAACATCGTGGAGCGCTACAGCGCCTTCCCGCTGGACGCCATCC TCAACGCGCGCCGAGCCGCGCGACCCTGCCAGGCTCACGACAAGGTCAAGCTCTTCTGCCTCACGGACCG CGCACTGCTCTGCTTCTTCTGTGATGAGCCCGCGCTGCACGAGCAACACCAGGTCACTGGCATCGACGAT GCCTTCGAGGAGTTGCAGAGAGAACTGAAGGAGCAGCTTCAGGCCCTTCAGGACAGCGAGCGGGAACACA CGGAGGCGCTGCAGCTCCTCAAGCGACAGTTGGCAGAGACCAAGTCCTCTACCAAGAGCCTGCGGACCAC CATCGGGGAAGCCTTTGAGCGGCTGCACCGGCTACTTCGAGAGCGACAGAAGGCCATGCTGGAGGAACTG GAGGCCGACACAGCCCGCACACTGACGGACATTGAGCAGAAGGTCCAGCGTTACAGCCAGCAACTACGAA AGGTGCAAGAGGGGGCCCAGATCCTGCAGGAGCGGCTGGCAGAGACTGACCGCCACACCTTCCTTGCAGG AGTGGCCTCCCTGTCTGAGAGGCTCAAGGGGAAGATTCATGAGACCAACCTAACGTACGAAGACTTCCCG ACCTCCAAGTACACAGGCCCCCTGCAGTACACCATCTGGAAATCGCTTTTCCAGGACATCCATCCAGTGC CAGCTGCCCTGACCATGGACCCAGGCACTGCCCACCAGCGCCTGATCCTGTCCGATGACTGCACCATCGT GGCCTACGGGAACCTACACCCACAACCACTGCAGGACTCACCAAAGCGCTTTGACGTAGAGGTGTCGGTG CTGGGCTCTGAAGCCTTCAGTAGCGGTGTCCACTACTGGGAGGTGGTGGTGGCGGAGAAGACCCAGTGGG TGATTGGGCTGGCCCATGAGGCTGCCAGTCGCAAAGGCAGCATCCAGATTCAGCCTAGCCGCGGCTTCTA CTGCATCGTTATGCATGACGGTAACCAGTACAGCGCCTGCACAGAGCCCTGGACTAGGCTCAACGTCCGT GACAAGCTTGACAAGGTGGGCGTCTTCCTGGACTATGACCAGGGCCTCCTCATCTTCTACAACGCCGATG ACATGTCCTGGCTCTACACCTTCCGAGAGAAGTTTCCTGGCAAGCTCTGCTCCTACTTCAGCCCCGGCCA GAGCCACGCCAATGGCAAGAACGTCCAACCACTGCGGATCAACACCGTCCGCATCTAATCTGGGCAGAGG GAGACCAAAGCCTCCTGGGGCTGTGACCACCAGCAAGAGCCTTGCCTGACAGATGGAGGACCTGGACTGC TCAGGCCCACTGTGGCCACCCTGAGACCTCAGGCCAACTGTTTGTCCCCTCCTCCTCCCCTTCTGCGAGC TGGAGATTGCACTCCGTGGTTGATGCCCTGTGGCTCTGATCTTAATGGGATGTGACATGGCTTCTCCCCA GGGCAGCCCTTGCCCAACCCCTCATCCCCATTCTCTCAGGAACAGGGAAGCTCTCTGTTCCCCTCCTCCT GCCCAGCTAACCTAGCATCGTGACAGGTCAGATGTGATCAGTAGCTAGCAGCCCAAAAGGTATACAAAAT CCTCTTATGCCTAAGAATTGACTCACCCTTGACCAGGAGAGCTATGGGCCATTTTACTTTGGATCCAATT CCTTAGGGGAGCTTGGCAGAAGCTGGATTATTTGAGAGTCACCCTCTCTTGCTACCCGTGCTCCAAGCCG AGAACTGTGGGTTTCCTCCTCTCTCACTGATCTGCAAGTCACACAAGACAGGTAGCTCACAACATACCAT TACCTTCTAGCCCATGGATTCTGGGGCAGAGTGAGGTATAAGGACCCTAGAAACCCATATAGAAGAACAC CATCAGGACAAAGCACCAATGGATGATCCAAGTCCCCAACAGCCCCTGGGGACAGAGGTCCTACAATCCT CATCACACAGGTCTCTGACCTACTGCATGGACGGAGAGGGTGCTGGCAGCAGCTTTTTTTCCGTCAGGAC CTGAAGCCCAGGAGATTGTTATCGGGTACCACCCTGGACTTGACATCTGGCTCCCTTAGGATCCTGAGGC ACAAACAATAGCCGCCTTAGTTGACTTCATCAGACAAGAATTTGCAGTCAGCTCCCTGGTGACTTGCAAG ATCACTGAAAGGGATGGAAGAGGACAGCCTTTGCCAGAGTTTCCAGGAACAGCTCCCACCATCTAGAGGG TTTGTGTCTTGTGACCAGAATGGTGCTTCCATTTGCAGCTGCCCCACAAGCTGCTGGCTGCCACCATCCA CGCCAGAAAGTTATTGTCCGTGTCTGTCCCACCACACCACACCCCCCAACTCGGGTCCCAGTTCTACATC TTTCAGAGGGATTCTTTTTGTGGAAACTTGAGTCCAGAACCTAGACTTTGACTGCAAAGCACTCTGAGAA ATGTAGTTTTTCCTCTTCCAGCCTCAGCCACAAAGTTATCATGGAGGTGGAATAGATCCATCCACAATTT CCTTGCCAATGAATCCAACTGTGAAGAACTCAGGAGAATCCATGAATCCACCTGAAGGCCTTGGCTTTGA AACAGAACTGCTAAAAATGTCCTGCTCCGTGACTCTGTCCCTGCCTGTCCCTGCCTGTCCCTGCCTGTCC CTGCCCAGAGGCAACAACTTAGGGAATCAGTAGCATCAGAAATAGAGACAAAATGACTTCCCAGCCTGGG CCTTCCTTTGTTGCCCCAGGTCCTCAATTCTGTACTTGGGATTCAGTGAGCATGTCCATCTCCGGTGAGC CAGAATATGAACTTGAGGGGTTCTCACTCACAACTGCCATGTGCTAGCCCCCCCTGAGCAAGAAGAATCC TGAGTCCTGCTCTTCCTCTCAACTCCCAGACACCAGGCCACGCCTGGCAGTATTTTTATTTAAAACATGG CCCCTTTTGTGCATTCTGATCAGTTTGTATTAAATTAAAGTTACAGGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416690 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416690 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7640 same embryo
	EMAGE:7642 same embryo
	EMAGE:7638 same embryo
	EMAGE:7639 same embryo
	EMAGE:7637 same embryo
	EurExpress:euxassay_004737 same experiment
	MGI:4828918 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source