EMAGE mouse gene expression spatial database

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EMAGE:7737

Mrpl54 mitochondrial ribosomal protein L54 ( MGI:1913297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007426. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007426_01	euxassay_007426_02	euxassay_007426_03	euxassay_007426_04

euxassay_007426_05	euxassay_007426_06	euxassay_007426_07	euxassay_007426_08	euxassay_007426_09

euxassay_007426_10	euxassay_007426_11	euxassay_007426_12	euxassay_007426_13	euxassay_007426_14

euxassay_007426_15	euxassay_007426_16	euxassay_007426_17	euxassay_007426_18	euxassay_007426_19

euxassay_007426_20	euxassay_007426_21	euxassay_007426_22	euxassay_007426_23	euxassay_007426_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7737_wholemount_strong.wlz

7737_wholemount_moderate.wlz

7737_wholemount_weak.wlz

7737_wholemount_possible.wlz

7737_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7737_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 19 20 21 22 23 24

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 16 17 18 19 20

diencephalon lateral wall mantle layer

strong

spotted

strong expression: see section 12 13 moderate expression: see section 11 14

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 10 16 17

pons mantle layer

moderate

regional

moderate expression: see section 08 18 weak expression: see section 07

midbrain ventricular layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

ventral grey horn

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 14 15 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 16 17 18 19 20

palatal shelf

moderate

regional

moderate expression: see section 07 08 09 16 17

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24 moderate expression: see section 06 07 20

clavicle

moderate

regional

moderate expression: see section 08 09 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9069
Entity Detected:	Mrpl54, mitochondrial ribosomal protein L54 ( MGI:1913297)
Sequence:	sense strand is shown >T9069 CGAGCGGCCGGCCAGCGTCATGGCGGCCGCCCATCTCCTCCGGGCTTCCCGGGTGTGGGCCCGCTGGCAT CCCCGGGCGCTCCCGGTCCTCAGAAGACCTGGAGGATTCTCTATCCGGGAATATGCTAAAAAGCCAGTTG GCAAGGGCGGCAAGGGCGGCGTGGCCGCTGAGGCCCTGAAGGACCCCGAGGTGTGCACAGACCCCACTCA GCTCACCACACATGCCATGGGGGTCAACATCTACAAGGAAGGCCAGGATGTGGCCCTGAAGGCAGACTCC GAGTACCCCACATGGCTGTTCCAGGTGAACTTGGGTCCCCCCAAAAAGCTAGAGGAACTAGAACCAGAGT CCCGAGAGTACTGGCGACTACTTCGCAAACAGAATATCTGGCGCCACAACCGGCTGAGCAAGAACAAGAA GCTGTAGTGTGATGATGGGCACCTGACGGGAGACTGCCTGGAACCCTGGCCAGCCAGAGCAGGGAGAGAA CTGAGTCCTCTGACCTTCACCTGCTCGCCATGCCGTGCAGGTCATGCACACTCGAAATAAATACCAAAGG GACCTACAGCAGCCACGCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6818054 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6818054 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7738 same embryo
	EMAGE:7740 same embryo
	EMAGE:7739 same embryo
	EMAGE:7736 same embryo
	EurExpress:euxassay_007426 same experiment
	MGI:4826451 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source