EMAGE mouse gene expression spatial database

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EMAGE:7821

Ryr1 ryanodine receptor 1, skeletal muscle ( MGI:99659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007404. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007404_01	euxassay_007404_02	euxassay_007404_03	euxassay_007404_04

euxassay_007404_05	euxassay_007404_06	euxassay_007404_07	euxassay_007404_08	euxassay_007404_09

euxassay_007404_10	euxassay_007404_11	euxassay_007404_12	euxassay_007404_13	euxassay_007404_14

euxassay_007404_15	euxassay_007404_16	euxassay_007404_17	euxassay_007404_18	euxassay_007404_19

euxassay_007404_20	euxassay_007404_21	euxassay_007404_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7821_wholemount_strong.wlz

7821_wholemount_moderate.wlz

7821_wholemount_weak.wlz

7821_wholemount_possible.wlz

7821_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7821_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 21 22

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 16 17 18 19 20 21

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20 21 22

shoulder rest of mesenchyme

moderate

regional

moderate expression: see section 21

foot mesenchyme

moderate

regional

moderate expression: see section 02 03 16 17 18

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 20 21 22

not examined

regional

not examined expression: see section 02 03

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 11 weak expression: see section 07 08 13 14

pons mantle layer

weak

regional

weak expression: see section 06 15

tongue muscle

moderate

regional

moderate expression: see section 09 10 11 12 13 14

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35290
Entity Detected:	Ryr1, ryanodine receptor 1, skeletal muscle ( MGI:99659)
Sequence:	sense strand is shown >T35290 GGGGTGAACTGGAAGTACAGAGAGTGAAATTCTTGAACTACTTGTCGAGGAATTTCTACACACTGCGGTT CCTGGCCCTCTTCCTGGCCTTTGCCATCAACTTCATCTTACTGTTTTATAAGGTCTCAGACTCTCCACCA GGGGAGGATGACATAGAAGGTTCCGGTGCTGGGGACATGTCAGGGGCAGGGTCTGGTGATGGCTCTGGCT GGGGCTCCAGGGCCGGCGAGGAGGTAGAGGGCGATGAAGATGAGAACATGGTGTACTACTTCCTGGAGGA GAGCACCGGCTACATGGAGCCTGCCCTGAGGTGCTTGAGCCTGCTGCACACGCTGGTGGCCTTTCTCTGC ATCATTGGCTACAACTGTCTCAAGGTGCCCCTTGTCATCTTCAAGCGGGAGAAGGAGCTGGCCCGGAAGC TGGAGTTTGATGGCCTCTACATTACAGAGCAGCCCGAGGATGATGACGTGAAGGGACAGTGGGACCGCCT GGTGCTCAACACGCCGTCTTTCCCTAGCAACTACTGGGACAAGTTTGTCAAGCGGAAGGTTCTGGACAAA CACGGGGACATCTTTGGGCGGGAGCGGATTGCGGAGCTGCTGGGCATGGATCTGGCCTCTCTGGAGATCA CAGCCCACAATGAGCGCAAACCTGACCCTCCACCGGGCCTGCTGACATGGATCATGTCTATCGATGTCAA ATACCAGATCTGGAAGTTTGGAGTCATCTTCACAGACAACTCTTTCCTGTATCTGGGCTGGTACATGGTG ATGTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89651. Forward Primer - name:089651_F_cDNA_Ryr1, sequence:GGGGTGAACTGGAAGTACAGAG; Reverse Primer - name:089651_N_SP6_cDNA_Ryr1, sequence:GGACATCACCATGTACCAGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7822 same embryo
	EMAGE:7824 same embryo
	EMAGE:7826 same embryo
	EMAGE:7825 same embryo
	EMAGE:7823 same embryo
	EurExpress:euxassay_007404 same experiment
	MGI:4827845 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source