EMAGE mouse gene expression spatial database

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EMAGE:7836

Adam10 a disintegrin and metallopeptidase domain 10 ( MGI:109548)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007598. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007598_01	euxassay_007598_02	euxassay_007598_03	euxassay_007598_04

euxassay_007598_05	euxassay_007598_06	euxassay_007598_07	euxassay_007598_08	euxassay_007598_09

euxassay_007598_10	euxassay_007598_11	euxassay_007598_12	euxassay_007598_13	euxassay_007598_14

euxassay_007598_15	euxassay_007598_16	euxassay_007598_17	euxassay_007598_18	euxassay_007598_19

euxassay_007598_20	euxassay_007598_21	euxassay_007598_22	euxassay_007598_23	euxassay_007598_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7836_wholemount_strong.wlz

7836_wholemount_moderate.wlz

7836_wholemount_weak.wlz

7836_wholemount_possible.wlz

7836_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7836_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 03 04 05 06 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 18 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 18

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 17 18

inner ear

strong

regional

strong expression: see section 02 03 04 18 19 20 21 22 moderate expression: see section 05 06 07 16 17

lens

strong

regional

strong expression: see section 01 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 21 22 23

anterior naris

moderate

regional

moderate expression: see section 10 11 12 13 14

external naris

moderate

regional

moderate expression: see section 14

nasal cavity epithelium

moderate

regional

moderate expression: see section 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

naso-lacrimal duct

strong

regional

strong expression: see section 06 moderate expression: see section 07 08 16 17 18 19 20 21

liver

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35878
Entity Detected:	Adam10, a disintegrin and metallopeptidase domain 10 ( MGI:109548)
Sequence:	sense strand is shown >T35878 CATGCTGCTAGTAGTGGTCCTGAGCTCCTGAGGAAAAAACGCACAACTCTGGCTGAAAGAAATACTTGTC AGCTCTATATCCAGACAGATCACCTGTTCTTTAAATACTATGGAACACGAGAAGCTGTGATTGCTCAGAT ATCCAGTCATGTTAAAGCAATTGATACAATTTACCAGACTACAGACTTCTCCGGAATCCGTAACATCAGC TTCATGGTGAAACGCATAAGAATCAATACAACCTCTGATGAAAAAGACCCTACAAATCCTTTCCGTTTCC CAAATATTGGTGTGGAGAAGTTCCTGGAGTTGAATTCTGAGCAGAATCATGATGACTACTGCCTGGCCTA TGTCTTCACAGACCGGGATTTTGATGATGGTGTTCTTGGTCTGGCCTGGGTTGGAGCACCTTCAGGAAGC TCTGGGGGAATATGTGAGAAAAGCAAGTTGTATTCAGATGGCAAGAAGAAGTCATTGAACACAGGCATCA TTACTGTTCAGAACTATGGCTCCCATGTGCCTCCCAAAGTCTCTCATATTACGTTTGCTCATGAAGTTGG ACATAACTTTGGATCTCCACATGATTCTGGAACAGAGTGTACTCCAGGAGAGTCTAAGAACTTAGGACAA AAAGAAAATGGCAATTACATCATGTATGCAAGAGCAACATCTGGGGACAAACTTAACAACAACAAATTTT CACTCTGCAGCATTAGAAACATAAGCCAAGTGCTTGAGAAGAAGAGGAACAACTGTTTTGTTGAATCTGG CCAGCCTATCTGTGGAAACGGGATGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97299. Forward Primer - name:097299_F_cDNA_Adam10, sequence:CATGCTGCTAGTAGTGGTCCTG; Reverse Primer - name:097299_N_SP6_cDNA_Adam10, sequence:ACCATCCCGTTTCCACAGATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7835 same embryo
	EMAGE:7833 same embryo
	EMAGE:7834 same embryo
	EMAGE:7831 same embryo
	EMAGE:7832 same embryo
	EurExpress:euxassay_007598 same experiment
	MGI:4822971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source