EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7856

Adamts15 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15 ( MGI:2449569)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007606_01	euxassay_007606_02	euxassay_007606_03	euxassay_007606_04

euxassay_007606_05	euxassay_007606_06	euxassay_007606_07	euxassay_007606_08	euxassay_007606_09

euxassay_007606_10	euxassay_007606_11	euxassay_007606_12	euxassay_007606_13	euxassay_007606_14

euxassay_007606_15	euxassay_007606_16	euxassay_007606_17	euxassay_007606_18	euxassay_007606_19

euxassay_007606_20	euxassay_007606_21	euxassay_007606_22	euxassay_007606_23	euxassay_007606_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7856_wholemount_strong.wlz

7856_wholemount_moderate.wlz

7856_wholemount_weak.wlz

7856_wholemount_possible.wlz

7856_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7856_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 23 24

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 21 22 weak expression: see section 19 20

hindlimb digit 2 mesenchyme

weak

regional

weak expression: see section 19 20

hindlimb digit 3 mesenchyme

weak

regional

weak expression: see section 19 20

hindlimb digit 4 mesenchyme

weak

regional

weak expression: see section 20

hindlimb digit 5 mesenchyme

weak

regional

weak expression: see section 20

foot mesenchyme

moderate

regional

moderate expression: see section 20 21 23 24

femur

moderate

regional

moderate expression: see section 09

vibrissa

moderate

regional

moderate expression: see section 05 06 07 15 16 weak expression: see section 18 19

axial skeleton

weak

regional

weak expression: see section 11 12 15

tail mesenchyme

moderate

regional

moderate expression: see section 10 11 12 14 15 16

axial skeleton tail region

weak

regional

weak expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35886
Entity Detected:	Adamts15, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15 ( MGI:2449569)
Sequence:	sense strand is shown >T35886 GACCTTCCAAAGTGACCATAGCCCTGTACCCACTGAGGGGAAGTGGTTGTGCCTGGGAGGGTGAGGTGCT GAGAACCCACATGCTGGCAAGTCAGCCTTCTACTCTGGCAGCTCCTGCTGTCTCCTGTGGGCATGCTACA TTATGTCTCCCGGCCTTCAGTTCTCTGGTAGGTAAACGGGCTTCATTGTTTGCTACCTCAGATCATGCTC TCTAGGACAGAAGTAACCTGGTGCTCTCACCCGTCTCAGCAGGATGTGCTCAGCCACGGAAATAAGTCTG TGCGTGGCTTTCCTGATCCCATAGCCTGGCTATCAGCTGGGGCTCTAAATACAAAGACAGAGTCGGGTAG GGTATCTTCTTCCTCAGGTTCCTTGGGATCATGTTTTAGAAACACTTAAGAATCAGTACCCTGACCTTGG GACACTGGAGACCCATCTGTCATTCTTGTAGACATGGCAGTCAAAAAGAAATCCAGGTGTTTCTTATAGC AGGGTAATTCTTGAAGACTTGGAAGCTCCAATGTATATAGGACATCTCCAAAGACTCGGCAGTACATCCA GGACCTCAGCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81803. Forward Primer - name:081803_F_cDNA_Adamts15, sequence:GACCTTCCAAAGTGACCATAGC; Reverse Primer - name:081803_N_SP6_cDNA_Adamts15, sequence:CGCTGAGGTCCTGGATGTACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7854 same embryo
	EMAGE:7859 same embryo
	EMAGE:7855 same embryo
	EMAGE:7858 same embryo
	EMAGE:7857 same embryo
	EurExpress:euxassay_007606 same experiment
	MGI:4822984 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source